Quantitative Studies on Antibody Purification: I. The Dissociation of Precipitates Formed by Pneumococcus Specific Polysaccharides and Homologous Antibodies
Description:
Throughout the early 1930s, Heidelberger, Forrest Kendall, and Elvin Kabat continued their work to elucidate the exact chemical structure of antibodies, and namely to determine whether antibodies were modified serum globulins (a class of proteins in the clear liquid portion of the blood that separates out upon coagulation). In this article, Heidelberger and Kendall detail their technique for isolating pure antibodies by using varying concentrations and types of salts to break apart the antibody-antigen precipitates produced by the precipitin reaction. Once they had isolated pure antibodies and examined their nitrogen content and other chemical properties, they concluded that they were, in fact, a type of globulin, a group of proteins not soluble in water but soluble in salt solutions.
Item is a photocopy.
Number of Image Pages:
12 (1,019,451 Bytes)
Date:
1936-08 (August 1936)
Creator:
Heidelberger, Michael
Kendall, Forrest E.
Source:
Periodical: Heidelberger, Michael, and Forrest E. Kendall. "Quantitative Studies on Antibody Purification: I. The Dissociation of Precipitates Formed by Pneumococcus Specific Polysaccharides and Homologous Antibodies." Journal of Experimental Medicine 64, 2 (August 1936): 161-172. Article.
Publisher:
[Rockefeller Institute for Medical Research]
Rights:
Reproduced from the Journal of Experimental Medicine, 1936, 64, 161-172 by copyright permission of the Rockefeller University Press.
Subject:
Medical Subject Headings (MeSH):
Antibodies
Streptococcus pneumoniae
Pneumococcal Infections
Polysaccharides
Exhibit Category:
Antigens and Antibodies: Heidelberger and The Rise of Quantitative Immunochemistry, 1928-1954
