XI tt

INSTITUTE 6F MIGROJW3LUCiY

kcific polysaccharide L1 of Tne VI gnrwcoccuo.  Thh rubetoncs io being

stadis8 wi$& Da. Paul A. Sabers os part of R project, undertaken with Prof. M.

Btolcey and Dr. S. Pa. Barker, of as, Wniverrity of BiminghPm, England, to

crlucfdake the fine rtructztrso of thcs specific cstpsllrlra~ polyooccharider of Type8 II,

V, send VX pnaumococwr.  This knowledge is required m order to correlate the

otrtocturer of their typ-specific rntigens.  By initial cleavage of one of the

plmarphatta linkrga.r of the Type VI eubatmce with dilute alkali, foUowed by oplfttiag

of the other om mild acid hydrolyals, it has now apparently been poreibls to trolate,

in crystalline form, the phor)dhrLts*free repeating unit, which esaaistr of

of the regmating unit of a cmnplcsx polyracchratide io moot uaurwrl.

Croro raactionr of antipneumococcal #era.

The prediction could be mads

that if the ~roro recctiono of glycogen ia Type II antipeumcxoccal mra am

actually due to reaction with ontibody at the multiple u-1) 4,6..brurch points,

p0rr"tial t-QV4Ll of the large ma80 of OUtsr a- f Qqhcors chains Of $lyCQ#QSX by

means of phorpbclrylora asd, better, mwe sonrpilstc, removal by p-aanylarc, would

progrerrivsly inerasroe the mount of antibody predpitatsd.  Partly with the help

of rurrptiso furairhed by Dr. Joseph LarnQF, of the IhiVorSity of Uiiasts, there

predictions were conpl~stsly verified.  Rabbit oati+arrr tQ pneu#lococeai Type8 M

and XI1 were also Etudied in addition to horae: #era, and it was concluded that

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glycogsn urrd amylopectin precipitate Type XU antisera dro at fhdr a-i, 1, b-

branch pinto.  The linkage ccpncenned in ths cwrr reaction in Type IX aattsera

$8 probably Q - 1 * &, rinca thu smewt of sntibady precipitated ir unaffected by

changer in outer chain length.

The recant sl\aci$stioa of the fins structure af the upeclfic pdyrrccbarida

frem the bekituts, patmittad the prdiction that dl plyarrccha~idsr which contab

partial hydrolysir, anit verified in thio instaacer.  Mormvar, oat plog barley

8-glucano, in which cellobiam unitr had been surpscted because of their bubvior

ob the rkuBfsr with saoymer.  Mora tscsntliy CSUQ~OQSS has actually been irolatsd

by prrtiral bydrolyoir of the &UC&PIO, IQ that t&a oarological tort hoe again proven

a powerful aid to tfre organic

tha enspologist.

Q-W-4-w '?

Proferror Wea$dberger har besn in Europe for six m-rs

and lectures in $wadan, Noxway, Scotland, EagLaad md FXPLPC~, md carrying an
rcrsmarch at the Facult; de Pkmmaeia of tbsb Univereity of Pipxlr, where the crosa

reaction8 of r~8r irn6 of tha plysaceharidss aif six bfffsrent rtrdnr of Aardbecter

pierogsnsr in rlnQfpneumococcal &era were studad.  Dr. Heiddberger also received

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