NEW YORK UNIVERSITY-BELLEVUE MEDlCAL CENTER

NEW YORK UNIVERSITY COLLEGE OF MEDICINE

550 FIRST AVENUE, NEW YORK 16, N.Y.

DEPARTMENT OF BIOCHEMISTRY

OREGON 9-3200

November 11, 1957

Dr. Maxine Singer
Institute of Arthritis and Metabolic Diseases
National Institutes of Health
Bethesda 14, Maryland

Dear Maxine:

Thank you very much indeed for the slides and the copy of

your talk to the ACS meetings in September.  Although I had read the
abstract I certainly enjoyed reading the full talk with the beautiful slides.
This will help me very much in my lectures on polynucleotide phosphoryl-
ase.  The priming story would be very incomplete without the beautiful
work that you people did.

Regarding the preparation of polynucleotide phosphorylase,

we are still bogged down trying to get once more good active extracts
and ammonium sulfate fractions therefrom.  We do not know exactly where
the trouble lies but in the past few months we haven't had much luck and
have gotten preparations of low specific activity unsuitable for the further
steps.  I hope we shall be able to clear this up in the near fugure and,
once we are ready to go through with a preparation, I will let you know so
that you can come over and spend a couple of weeks with us.  It will be
a pleasure to have you around.

With best regards,

Sincerely yours,

SO: mak

Severo Ochoa