November 6, 1958

AIRXAIL

Dr. Paul Berg
Department of hlicrobiology
School of Medicine
Washington University
Saint Louis, Missouri

Dear Dr. Berg:

   Yesterday Dr. Heppel told me about his telephone conversation
with you concerning polynucleotide phosphorylase.
supply is rather limited but we are working up B mammoth preparation
and unless things go even more awry than they have up to now we'll
have a good supply soon.
now have.
ty is rather low.
polymerization. The source was 4. agile. Its designation is 5-27AR58,
10.5 mg. protein per ml, specific activity (exchange assay)  is 21.
may be more helpful to tell you that

cent of 0.05 mg. poly A in 1 hour at 37' (pH 8.2, 0.01 M %H).

concerning the action of polynucleotide phosphorylase on acceptor RNA.
Giulio Cantoni and Marianne Manago did a few preliminary experiments
last spring and Giulio and I are now continuing them.
Bergmann about these last week.

   Fred has sent me the procedure for E, coli acceptor RNA and I
rhould also thank you for your offer to send me some of that material.

   We will send the polynucleotide phosphorylase either later this

At this moment our

In any case, we'll send you some of what we

Although it has gone through my steps, the specific activi-

Nevertheless it is good enough to show a lag for

It

- *Oo5 ml, phosphorolyzed 50 per

20

Dr. Heppel told you that I have been carrying out some experiments

I told Fred

week or early next week.
special delivery, and we'll send a telegram so you will know when to
expect it.
enzyme you'd like to have.

We'll pack it in dry ice and send it air mail -

Please write if there is any further information about the

With very best regards,  I am

Sincerely,

MPS: peg

Maxine F. Singer