INTFRNAT IONAi, CONFERENCE OK R ECOXR I NANT DNA MOLECULES

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Asilomar Conference Center, Pacific Grove, California February 24-27, 1975

Sponsored by the U.S. National Academy of Sciences with financial support from
the National Cancer Institute or' the Eational Institutes of Health, and the
National Science Foundation.

    MONDAY
FEBRUARY 24, 1975

TIME SUBJECT S PEAKEK

8:30 Introduction: History, Aim of Meeting,

OutLhe of Task

8:50 Assessment of present technology,

joining methods and vectors

Ecology of Plasmids and Enteric Organisms

Ubiquity of organism, natural his tory,
plasmid ecology and introduction to normal
bowel flora

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9:lO

General problem o€ Salmonella infection in
man and domestic animals and the use and
misuse of antibiotics in medicine and agri-
culture, enhancement of R plasmid emergence

Plasmids which contribute to virulence or
colonization of enter ic spec i.es

D. Baltimore
(HIT - USA)

P. Berg
(Stanford - USA>

S. Falkow
(U. Washington - USA)

E. Anderson
(Colindale - UK)

H . Wi 1 liarns -Smi th
(Hought on Poultry
Station - UK)

1o:oo Coffee Break

Gene transfer 2
organisms and Staphylococcus

of both enteric

M. Richmond
(Bristol - UK)

Panel discussion on ecology and potential S. Falkow
.dissemination of E. coli strains

(Chairman)

12:oo Lunch

1:oo A Public Policy Perspective

H. Green
(The George Washington
Univ. National Law Ctr.)

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lionday, February 24, 1975 cont.

TLPIE S US JE CT S PFAKE R

c

4: 30

Molecular biology OE bacterial plasmids

6:OO


7: 30

Molecular biology of bacterial conjugation and
conjugative mobilization of plasmid and other
DKAs

Phenotypic expression of plasmids in bacteria

Some features of DNA uptake by bacteria

Dinner

Genetics and biochemistry of DXA restriction

Molecular cloning of DNA as a tool for the study
of plasmid and eukaryotic biology

Development of bacteriophage systems as cloning
vehicles

R. Novick
(Pub. Health Res. Inst. -

USA)

R. Curtiss
(U. Alabama - USA)

J. Davies
(u. Wisconsin - USA)

M. Fox

(MIT - USA)

H. Boyer
(U. Calif., San Francisco -

USA)

S. Cohen
(Stanford Univ. - USA)

K. Murray
(U. Edinburgh - UK)

    TUESDAY
FEBRUARY 25, 1975

8: 30 Panel discussion on experimental guidelines R. Novick (Chairman)

for novel recombinant prokaryotes (Pub. TIealth. Res. Inst: -

USA)

12:oo Lunch

4: 30 Plasmid-viral DNA Recombinants

Design, construction and value of new kinds of D. Nathans
molecules using SV40 (deletion mutants, trans- (Johns Hopkins Univ. - USA)
ducing viruses, plasmid-viral gene recombinants)

"Natural history of viruses" ; especially, are J. Sambrook
viral genes attached to plasmids a special case?

(Cold Spring Harbor Lab-USA

Adeno-SV40 hybrid DNAs as a case history; includ- A. Lewis

required for use in lab, c) results of risk
assessment experiments.

ing: a) NIH distribution policy, b) conditions (NIH - USA)

page 3

Tuesday, February 25, 1975 cone.

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TIME SUBJECT SPEAKER

6:OO Dinner

7:30 Comparison of SV40 and human papova viruses: D. Walker

pathogenicity, (cross reactivity and onco- (U. Wisconsin - USA)
genicity)

Infectivity and transforming capacity of SV40 D . Jacks on
4- SV40 DNA; use of recombinant DNAs to infect
bacteria vs. mammalian cells

(U. Michigan - USA)

Isolation and study of Rous sarcoma proviral     M. Bishop
DNA presence of "virogenes" in "normal" cells    (U. Calif., San Francisco-

USA)

Possible experizents with herpesviruses DNA B . Roizman
fragments (U. Chicago - USA)

Panel discussion on guidelines for virus- A. Shatkin (Chairman)
plasmid recomb inants

   WEDNESDAY
FE'BRUARY 26, 1975

8: 30

- Plasmid-cell DNA Recombinants

What has already been done in linking cell and    D. Hogness (Stanford - USA)
plasmid DNAs                        D. Brown (Carnegie 1nst.-US

D. Carroll(Carnegie Inst.-

USA) i

10 : 00 CofEee Break

Major problems which can be solved by making     S. Brenner (MRC - E),

cell DNA-plasmid-cell DNA recombinant P. Day (Conn. Ag. Exp.     I
                                 Station - USA), R. Valen-
                               tine (IT. Calif., San
                                 Diego - USA)

12:oo Lunch

4: 30 Panel discussion of guidelines for plasmid- D. Brown (Chairman)

cell DNA recombinant experiments.

6:OO Dinner

7:30 Brief analysis of responsibility of research D. Singer (Fried, Frank,

scientists and of risk balancing. With touch on Harris, Shriver and Kampel-
ethical issues insofar as responsibility is con- man, Washington, D,C.; Hast.
cerned, and focus on the serveral components ings Inst. of Society, Ethi,
involved in balancing the risks and benefits
of creating recombinant DNA molecules

and the Life Sciences-USA)

Wednesday, February 26, 1975

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TIME SlTB J ECT

SPEAKER

Legal liability of investigators and institutions R. Dworliin
in the event of proximate or remote injury arising (Indiana Univ. - USA)
out of work with recombinant DNAs.
of Occupational Health and SaFety Act.
consent, insurance and indemnity.

Applicability

Informed

Institutionalization of current and future experi- A. Capron
mental guidelines, role of NIH Advisory Committee, (U. Penn. Law Sch. - USA)
international co-ordination of policies of
different nations.

THURSDAY

FEBRUARY 27, 1975

8: 30

Discussion and adoption of conference statement

12:oo Ajournment