3)
2)
3)
4)

5)


6)

1st Cloning 0 2nd clonj, '

celi dcsigm'Xon;.

bkdia used :

passed kn agar
      1 s t

2no
3rd

1-3) fncubated'wPt;'n . . ..rl. of . . . ..trypsin

14) spin  iQEz,rpz . . . . . . . for . . . . nfn. c$ 9~
                 IO       'C

75; resuspended
(