b-+. e
   Cell Designation....... d . . . . . .

   Ampole designation...;...
         h +-a., . . .

   date of freezing.. 1'2 3 3047
                            ..,.......,.

1) date of primary culture
                             I
2) origin of cclils         /       _~__.


                 I          /
                              I

T. Amano

Box #..I...?.%.!.....             ." ..,
        Total'!IOof vials

I             . ..- . . . . . I..........

Passage 41 2
          ..L . . . . .

1. :. . . .

I    I
I
I  I -`:
I

3) date of last subculture !   ,cefl f per gla$k
                                            I
'Media ~IZ(b'II~~~ j   Horse serum i  132s i&Y&' p sz\
date of treezing \ /C-V % confluent'   i &7) ;
                  color of media

Trypsin used ( sol A       I                   I
             /I - %'in media..L.....[.( sol.#

Centrifugation ,""'pL' /O?.i!L .j .i- i '_
Suspended in   ./.s>. ml of Media (
                        t       j..Cj
                                                        i'.

ml of 'med ia i

I
s; 0.
t
I

I
I
/    1

                     : ..~_ i ~~ :
at   . . . . ..c
                               I

type of flsk

i ml

,-20-C )
   .__- -' ,   - .  ~_ / ~.2q::hours _~ ~/ a.m.\ p.m. :             .

                                                                          -_  a.m.:, p.m.
                                            /       /       ,
                               ,
-170 c                     [--
                    !......hour& 1 a,m.l n-m. ,        s-m-`. n-m-
         .

Re'marks: ,- i     .*

                   - -- -.--.-. '-..---~.+-.. i .--I ~~
Numb&,-of vials$aken......i.. by..... j.....+.  date     .-
                                                          . . . . . . . . . . . . . . . . . . . .
                                                 ,


Datc pjji- /o'(;
     .*.* . . . . .

Cells In Freezer

-)Ccll designation........  .b............


2)date of primary culture
2 )origin of cells..............
)datc of the last subculture..... . . . . . . .
            ? ??? o
5)date of last mcdiurn change . . . . . . . . . . . . . .

6)

7)

8)

9)

11)

6

/


/


/

Kcdla Rlz
Es   2 :o;," ( w+c-K)
    $ lL#
cell morphology
?l oonfluenoy
pH color

Trypsin used  (',C i- $ In, .Q.;  lot#             lsadc . . . . . . . . . . . . . .
                                sol.# . . . . . . . . . . . . .

Zcntrlfugatlon  i c` -r-t   rpm I "       min.

suspended    !c': in                    $ lot#  1 b $ DIG0

                                c R loti?     lot# .."#.I...

/  ;- jiz lot$  $oGIimrln
                              ..* . . . . . .

             date of steril
             autoclared.

cell oount        /ml.      total  / bw-c
                                        o ??????????

Frcczlng method      . . . . .  Efl.  of ampoulas
                          P
freezing rate     C/rroin. 12) #i anpoule #
4%               a.rm                 . . . . . . . , kC.
              p.Xi.

In freezer or

-20%

a.m
p.m.

. . . . . . . . hr.

-170oc

a.a.
p.m.

. . . . . . . . hr.

in freezer

in freezer

Rcmarkst

                   3-
?f& 13) Box#   .>2 I'
                . . . . ..*..........