I%lTHSONlAN SCI  NCE INFORMATION EXCHANGE
    E                   U.S. DEPARTMENT OF       PROJECT NUMEA
`ROJECT NUMBER Do NOT use this space)  HEALTH EDUCAT I OPI AZD WELFARE
                    PhLiC HEALTk SERVICE
                                   NOTICE OF
                          INTRAYURAL RESEARCH PROJECT   ZOl HL 00014-01 LBG
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PER IO0 COVERED
July 1, 1975 through June 30, 1976
rlTLE OF PROJECT (80 characters or less)
Storage and Release of Molecules Required for Synaptic Communication

UAMES, LABORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER
PROFESSIONAL PERSONNEL ENGAGED ON THE PROJECT


  PI:    Marshall Nirenberg    Chief, Lab. of Biochem.
                           Genetics               LBG NHLI
  OTHER:  Richard McGee         Staff Fellow              LBG NHLI
      Saburo Ayukawa       Visiting Fellow           LBG NHLI
      -Clifford, Christian    Special Fellow            BB NICHD
      Phillip Nelson      Chief, Behavioral Biology
                           Branch                BB NICHD








COOPERATING UNITS (if any)

Behavioral Biology Branch, NICHD

.AB/BRANCH
Laboratory of Biochemical Genetics
FETT I ON
Section on Molecular Biology
INSTITUTE AN0 LOCATION
NHLI, NIH, Bethesda, Maryland 20014
IOT AL MANY EARS:         PROFEsSI ONAL:           OTHER:
  2.2                  2.2
SUMMARY OF WORK (200 words or less - underline keywords)
   The objectives are to devise biochemical assays for neurotransmitter
release from neuroblastoma and hybrid cell lines and then to define the steps
which are required for neurotransmitter storage and release and factors which
regulate these reactions.









PHS-6040
(12-75)


ZOl HL 00014-01 LBG

Project Description:

Major Findings:  The results show that the uptake of 3 H-choline into

neuroblastoma x glioma hybrid cells, the rate of acetylcholine synthesis and
the storage of acetylcholine are regulated by she conditions of cell growth.
The results suggest that the evoked release df H-acetylcholine can be ob-
tained but further work is needed 'to clarify the release process products.
The results also showed the presence of a dopamine storage mechanism in some
cell lines.

  Proposed Course:  When the assays for transmitter release have been
validated, they will be used to determine the reactions which are required for
transmitter release and to determine whether the steps are regulated.

Publications:

1. Breakefield, Xandra O., Neale, Elaine A., Neale, Joseph H. and Jacobowitz,
David M.: Localized catecholamine storage associated with granules in murine
neuroblastoma cells.  Brain Res. 92: 237-256, 1975.
                                     -

2. Rotman, Avner, Daly, John W., Creveling, Cyrus R. and Breakeffeld, Xandra
0 ' Uptake and Binding of dopamine and 6-hydroxydopamine in murine neuro-
b&toma and fibroblast cells.  Biochem. Pharmacol. 25: 383-388, 1976.
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