;MITHSO:JIAN SCI  NCE INFORMATION EXCHANGE     U.S. DEPARTMENT OF
'ROJECT NUMBER Do NOT use this space)                         PROJECT NUMBER
                        HEALTH EDUCATION AN0 WELFARE
            Pfi8LI;oCH;;~T&SERVICE     zol HL 00003-03 LBG
                        INTRANURAL RESEARCH PROJECT

PERIOD COVERED

July 1, 1975 through June 30, 1976
TITLE OF PROJECT (80 characters or less)

Regulation of Receptor Activity

UAMES, LAB~RAT~FIY ANO INSTITUTE AFF~L~ATI~Ns, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER
PROFESSIONGL PERSONNEL ENGAGED ON THE PROJECT

PI:    Marshall Nirenberg    Chief, Lab. of Biochemical
                          Genetics         LBG'NHk
     Hiroshi Matsuzawa     Visiting Associate          LBG NEIL1

COOPERATING UNITS (if any)

None

.AB/BRANCH

Laboratory of Biochemical Genetics
;ECTION

Section on Molecular Biology
INSTITUTE AND LOCATION

NHLI, NIH, Bethesda, Maryland 20014
TOTAL MANYEARS:            PROFESSIONAL:           OTHER:
    1.3                  1                   .3
SUMMARY 0F WORK 200 words or less - underline keywords)

Evidence for a new type of PGEl receptor coupled to cGMP accumulation was
obtained.  Cell lines with PGE
as cell lines with 2 species  o $ receptors coupled only to CAMP were found as we3
             PGEl receptors, one coupled to CAMP accumulatior
the other to cGMI? accumulation.  The 2 species of PGE 1. receptors also desensitiz
at different rates.  These results show that the coup mg of PGEl to increases i
cAMP and cGMP levels are clonally inherited properties which can be expressed
independently.

tws-bu4u
(12-75)


ZOl HL 00003-03 LBG

Project Description:

   Objectives:  The objective is to define receptor-mediated responses of
clonal cells which can be used as model systems for synapse studies.

   Major Findings: Evidence for a new type of PGEl receptor coupled to &HP
accumulation was obtained.  Cell lines with PGEl receptors coupled only to &fP
were found as cell lines with 2 species of PGE  receptors, one coupled to CAMP
accumulation, the other to cGMP accumulation. fn, e
also desensitize at different rates.       2 species of PGEl receptors
                      These results show that the coupling of
PGE  increases in CAMP and cGMP levels are clonally inherited properties which
can lb e expressed independently.

Publications:

1.  Matsuzawa, Hiroshi and Nirenberg, Marshall:  Receptor-meeiated shifts in
cGMP and CAMP levels in neuroblastoma cells. 'Proc; 'Natl; `Acad; Sci; USA 72:
3472-3476, 1975.                                                             --

2.  Bachrach, Uriel:  Cyclic AMP-mediated induction of ornithine decarboxylase
of glioma and neuroblastoma cells.: 'Proc; 'Natl; .Acad; Sci; 'USA.72: 3087-3091,
1975.                                                              --

2