Yale University New Haven, Connect&$ oGpo

DEPARTMENT OF BIOLOGY
Kline Biology Tower

March 17, 1971

Dr. Marshall Nirenberg
Chief, Laboratory of Biochemical Genetics
National Heart and Lung Institute
Building 10, Room 6D18
National Institute of Health
Bethesda, Maryland 20014

Dear Marshall:

  It was good seeing you and hearing about your work with the
neuroblastoma line at the NRP Conference.  John Minna expressed an
interest in learning about our techniques for identifying cell line
specific chromosomes utilizing heterochromatin staining techniques.
Mr McMorris has sent to him a preprint of an article in press on
this subject by Ruddle & Chen.  We have also sent him our protocol
for visualization of phosphoglucomutase electrophoretic differences
between Neuro and L-strain cell populations.

  As you know, Art McMorris in our laboratory has some hybrid
cell lines between neuro and mouse L cell lines.  We are particularly
interested in testing these hybrid clones for differentiated functions -
especially neuro associated enzymes.  It would be a great help to us if

send these, I can stop by possibly next Thursday and pick them up.
           *'.'- I. '." . . . . . ..__. __ ,__ ^,. ,         . ..__.

Yours sincerely..   

FHH:ItllT

Frank H. Ruddle

cc:  John Minna

cc:  F. Arthur McMorris