Lecture, Februnry 1, 1954

  Changes in state of AC -- the effects;  Braage-fusion-bridge
   cycles in endos?erm;  Tr,ansposlt?on of Ds (BP-gin),
I. In previ.ou.9 two talks, discussed the disappearance of AC from a kwwn
location and its ap?>erance at a new location -- transposition of AC as
explanation given.

Also discussed the origin of changed action of AC:

1.
2.
3.

Double dose
Increase in
Decrease in
  break at

action of single AC

action of that previously present but not doubled,
dosage action -- one dose produces an earlier timed
7)s than that produced by AC it replaced.

II, The c-anges in state:  Besides (E), (Z), and (3) above, have other
   dif_'erences arising.
   AC in double dose:    These related to action of a sLngle AC or an
                Control of the time of Ds breaks can be very
   precise.

III.  The relation of changes at AC to those at Ds:  A high rate of
coincidence.  If a break occurs at Ds, then a chanse at AC is quite
likelp to be detected,

IV. The nature of the patterns T?roduced by doses of AC and various states of
  AC:   Begin Jan. 28 outline:

Add: Stabilized AC action:
             --.-.. ^ . .  -.-.w-". I____-
             -L _,---. _, -_ ."arl,.YX_L_ _ .., ..-" ."vs,
     C sil bz wx ds ac female x I Sn Bz I:x Ds   Ac-stabilized,

Vi The transpositicn of Ds.

1. Xow such transpositLons detected:   Those occurring from one
position to another in the short arm of chr mosome 9 easily found in
the tests with Ds.

2. If

a>.

b).

CL

Ds moved from standard location to positton between Bz and bix:

The breaks:

I Sh &z    Dti       l`?fX      ,66     . ..C

Ds break:  nroduces
Prophase:        a dicentric chrcmatid and acentric fragment:

           I Sh Bz            IdX
              IC                     .- ----o-----

                                                                    "
           I Sh Bz             WX

The following anaphase:


Break at (1 1

The 'telophases

Break a t

%v- n
    "                -2 ilx
                                                         `--t -------

The

of  sectors expected in the C sh bx areas:   Wx with wx spots,
          z< le A. ;,I ."-c-

3. If Ds moved to a position between I and 77:   1 aw>~ co+-

                                           ?-            IA%.
                                           +    SR;

    Ds break:   Prophase   3 ~~~&.-.+L .- -- -------- .
                              I    EQI 9  iw -i:

Anaphase of divisicln following


4. If Ds inserted to left of I:

    Ds I Sh Bz      WX
   -----------------
    I: ShS,        w

VI, Analysis of a case of transposition of Ds from standard location to
     position to &#@&t of I,

1. Cross in rGich detected:   C sh bz wx ds ac female x I Sh F3z Xx Ds AC

Tne ksrncls on the ear:  Table 1

2.  The ker;nel showing 13s to left of I:   Ds I Sh Bz wx    AC
                                ds C sh bz FIX   ac

3. Plant from this kernel grown;  crossed to a number of tester plants
   including females, C sh bz, and either- Wx OP wx, ds, ac,
(a). T-Des of kernels on resulting ear:

Ds I Sh Bz AC
ds C sh bZ ac

Table 2,

(b). Same :?lant to females, c sh Bz wx ds ac:   Types of kernels:

       Table 3.
               s

4. The variegated kc-nels from crosses to C sh bz Wx ds ac females
    selected and plants grown from them.

(a) Crossed to C sh bz wx ds ac females.   The types of kernels on
       ear:
      Table 4.
              -_-

(b) Cross .d to c sh Bz wx ds ac femalesc  li'he Qwes of kernels on
          ear:
      Tse so



TbTd = 113s

  mar:s63C.



= 39

L ah

t lb3

= I37

=  Y



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