Continuation or" Trnnsposition of Ds;  Locations of Cls in shod 37-m 0P
  ckrrc;~o scrle 9 o  The missing regions;  ??? o  The states of Ds,

I.  Begin again, Trans-:3ositTon Ds 4759.  page 3 of Egbruary 1 discussion.
                                                        _
II. A secnnd illustration - Ds lt710,

1. Origin: in cross 03

c sh Bz wx ds ac feale x    C Sh Bz W;: Ds
              fj & Bii W-X ds   Ac     nale
                                                        ac

Apnemance of
-_     kernels on ear --'Table:
             G    7 f&j-j (,I --$#+I& QQ q-j \D w-J&-J.
                                 c__

2. Bariegation exhibited by odd kernel:  Breaks between Sn and Vx:

? o                                                              C
   C Sh irlx with c sh Wx areas.  Wx suba:-eas in/Nx sectors.
b).      Places Ds to left of Yx.
   No twin deep C - c areas.   Places Ds to right of C

3. Plant grown from komel in greenhouse.  Presumed consit1ltion:
                                    -...
        C Sh Ds 11x AC
         c shdswx ac

   Position w<th regard to Bz requi?ed deter&:-ation,
4. Greanhouse plant crossed to:

(1) c sh Bz ICC ds ac plant:  Showed Ds between Sh and Mx.
(2) C sh bz pm ds ac 177 ~TI'~:
                             ___A...
    Betrrcen Bz and Xx.   Ckotred Ds to riF$t of 3~:

so Tables will be given frown cross;'s
    surll^:er.               riade in Pield the follo:rin;_


,' !A). Va?xSegai;ed ki?:nels selected from :TOSS of croetiouse plant to
      C sh bz wx ds ac rllant:

i,
\
i
f

;'resmed cons%i.tx.?tion:  C Sh Bz Ds Wx      AC
                                                      -_I
                   C sh bz ds wx        ac

Crossed tc C sh bz wx ds ac female plants. m:

Crossed to c sh Bz wx ds ac female -lants,

\,    Ii ot crossed to c sh bz wx ds ac fexale plants as tXs stock not
        avaIlable at tine tests T,::re xde.

6, Coq?,~.r~isons of vnriec;ated ke?nels from all of the .c??osses:
             Table on board: @ L
                           ^-         qaq a+!    "& @J W/B

     Selection of one of the two c::osso:3er classes in each cese,
The- ,eforqe, the ra-bios give the cross-over :3ercentages:
                12

Sh Ds   WX
sh   ds wx.

The j2! Sn j8# PJx Nx AC kernels:

11

113%

   sh Ds Nx kernels (AC>:     135     9,137:

2   Sh Ds xx; AC kernels :     2G   13,875

                        23$ the normal amount
                               between Sh and !;lx.

The position of Ds4.710:

Sh           Ds                 WX

    1            :   L!2

                        4Q. 6rlQ+~W
g1.   The position wh;>:?e Ds has entered and been studied:  Non-random,


L              I ShBz                  1;TX
                                            ,


2, Positions befxeen I and Sh:

3 cases

2 cases
J'ust to left of Sh

3. Keasons why Ds not seen in other positions: Produce ei.the17
   lethal condition in ketcrozygoke -- dominant lethal, or
   very xw.5~ reduced viability.

a). Evidence from obsorved trans;:o$itions between Bz and I;x -- xany
    @.?;e defective kemels,   Soxe give defective erbryos; soze
   'give no?xml kernels but these do not gerxi~ate,

b). Kernels f~orn abscut half of the cas.cs of  t~~ms7bsition of tis to
   new positioils did not give plar:ks even when-- they ap>:,e:-:?md to

d.   be normal,
  Study of eff: cts of Ds dnen loccted between 1 and Sh -- n:>oduces
   dominant leth,als or low viabilities in k-Lc:rozygote --- t~i.11
    be discussed later.

4, -pat about cases  of im::rtion of Ds at known loci?   Produce m.t nb le
  ' genes.
  discussed, %-is must be postponed until anot;:er aspect of ;>s

IV.  V&at hap?7ens to Ds at the standard locsticn?when transpositions occur?

  1. Ca:Jes of no Ds at star,dard 1ocati.m:  I Sn Bz I:Tx Gs to
           Ds I Sh Bz Wx   No i)s at stendzrd location,

  2, Ceses of BBS at standsrd location:  Two t:F:;e s :
              1
      Ds I Sh Bz \?x Ds2 - both DS'S give f::e~~ue~t b?n&s.
              1
      Ds I Sh Bz Vx Ds2-  Ds'<gives many breaks but Ds2 behaves
           quite differently.

3. The nature of the diT'ference in behavior of the tme types of Ds
          will be discussed.  kroortant for our understanding
        of mukble loci and their action,

v.  Tne changed states of Ds.

1. The first case of recognition of altered Ds action,   The
    cross:

C sh bz wx ds ac female x I Sh Hz wx Ds     AC   male
                    C sh bz wx ds   -2iE


2.  The pattzm of vasiegation exhibited by mjority of kernels:
      Like that of photographs 1"rhen 1 AC present,

3. 1x7 all tests -- get sectors on sane of the kernels:

4. Occasionally f a whole kernel has C2.s t:;Te of pattern.   What has
  changed?      s it Ds ? Is it AC?

5. The tests to discover this:

a). Kcmels taken off ear; plants grown from 9-t;.  Crosses to
   C sh br. wx ds ac femaies.   The same pattern ap-xcrs in
     aariegated k crnels ala that fron w%ic?: Islant amse,

Crosses to c sh Bz wx ds ac;   Crossove17 classes wii31 C and 1~s:
Ike pattern in the C - c kernels:

Sacie as $hat from r&xi-c% plant allose,

Crosse d to I Sh Bz lifx Ds no AC.   The constitution bZ' the
    kcxels:

I Sh Bz ifx Ds / I Sh Bz wx iiD~'f     1 AC.
    Conside:.lable arnoEnt of' vzriegatlon -- wx

b), Ke:?ncls fro:2 the I Sh Bz Wx ils / 1 sh Bz wx 'rDs'* with large wx
       areas Temoved.  Plants grown frou them, crossed to
     C sh bz xx ds ac female plants.

The t+les of Ircxcls on the res1:lt:i.ng ears:   Table:

I Wx, non-variegated
1 wx,             425
    non-vx3egated 412

I Vx var.  usual pattern  361.
I Wx var.  new pattern    3&t

I wx var.  usual pattern 10
I wx var.  new pattern  364


CL Conclusions:  It is not the AC factor that is responsible for the
     altered pattern.  Ds in the Wx carrying chrc-losome be?isved
    normally with regSwrd to pattern.  Like the& in the Plant
    that gave rise tc the original aberrant kernel.

(2).  It is the Ds in t?e I ah Bz wx chrr;moscne that is altered,,

(3).  The 10 kernels in the I wx class showing the regulnr pattern
   prob::bly arose from crossing-over:

(I!.) .  The 34 kernels wit5 the abw pattern prorbably arose from
      sev.5317al caases:

(a>.  From crossing-over

(I-d. iiew changed in the Ds in the I Sh Bz Wx Ds Chromosome.
(c), Possible changes in action of AC,

V, m T,bsts of the stability of the new pattern of variegation produced
  by the altered Ds.

1. The original change from regular pattern to new nattcrn comes fn
one step:  The few kernels w?th these patterns in crosses and the
a- nenrance of an occasional sector suggest t3s.

2, Can the Ds change back to the original type of varieg.ation pattorn?

3. The test methods:    I Sh Bz Xx or wx Ds-altered pattern carrying
   plants crossed to C sh bz wx ds ac plants,

    (a). Kernels on ears examined fo r        Y     o
breyks.   Few found.             any snow~zg a hli&:~r frequency of
           lAhese removed from ear, plants grwim from the.

    b). Thec,e plants, in turn,
~~ajoritg of variegated kernels    crossed to C sh bz wx ds ac plants.

plant.               on ear now lmke that x?Zc':: gsve rise to

selected, A few kernels with slightly more variegation,
     plants grown from them,              '%ese,
                                         an d                  in turn,
                                                 c: r
plants.                        or,ses ma-:e tc C s`n bz wx ds ac
  cc). Again, major5ty   variegated
            of/kxnels on ear sh ow the pattrn given by the
kernel from xhhlch the plant a1=ose,

  (d). It required about 3 generations o.f careful selection to get a
Ds that behavbd like the original Ds tl1a.t produced t?e f-l. pattern.

4. Tnese diffcrcnces in the behavior of Ds -- altered states of Ds,

VI.  Tne meaning of t.ixcse states is very im-ortant and will be muc'n
clarified w??en the mutable genes are considered.  Before doingnt?:is,
will Uiscuss, brifely at beglnnin,? of next period, the mctnod 01 transposi-
tlon of Ds.,



  =




   z





Z




,c







  Z




-





=




=







  =



  =

16s

r)7
=

5

I

3



lj6 73-- R - a


  ka,
- ..;,-.      .

43

       `l\r8n
L--- -.-- ___"__. ._

.o

9

0

32

I

33'
1-v

30

             OJeL
-. ._ .,

3


                     i
,


.


dot

-  -. _.- I.  . ---. -  _.-._  _.I _

.

da       Lt "Is-


  .- --.. -_-...   ,...                




---- --__.                                      : .