The "A2 t'           m-l
    state of 22 .

The expression of linkage of Spm with the wx locus in
         chromosome 9.

I. 8731B-2 x 8726-2

The fully pigmented kernels have no active Ypm in the
endosperm.   The colorless kernels with pigmented spots reaeived
an active SJ& from the ear parent.   The apparent linkage of this
Spm with the 3 locus is 9.5% (42 "crossovers" in a total of 439).
xhe linkage may be closer than these data suggest.   Some
transpositions of SJJ= may have occurred in the ear parent before
the meiotic divisions.   Meiotic segregation could then produce
a gamete carrying the &c locus and m,  the association not being
related to crossing over.

II. 87353-l tiller ear x 8726-2
The pericarp variegation is an expression of the "A2" state
of a2m-1.  Anthocyanin pigment is absent in those pericarp cells
that have an active Spm.  It is produced in those cells having
no active Spm, and the intensity resembles that given by the
                           .`rr
standard A2 locus.                   \ ! b',lfy .+
       ('@i@$ent intensity,.\'is hlways greater on the
                        !-..'c
sides of the kernel than on the top.  See photo.  of self-pollinated
ear of plant 87353-l.)

Linkage of Spm to the wx locus is close,  the apparent crossovers
being 11 in a total of 253 kernels or 4.3P0

III. 87353-5 self-pollinated.   This photograph shows the
phenotypes of kernels that appeared on the self-pollinated ear
of a plant having one active Spm,linked to the 9 locus,  Among
the 245 kernels on this ear,  the following is evident:
(1) Normal ratio of Wx : wx = 181 Q : 64 9 ($= 61%)
                               - B
(2) One Spm in parent: Ratio of "No Spm" (fully pigmented
    kernels) to kernels with active Spm (colorless kernels
     with pigmented spots) is 66 : 179.         .' , f-.' :') s;, .
                                                  II./ j
(3) Linkage of Spm with wx is expressed:
          117 in the Wx class to 62 in the wx class.
                                                                 -
(4) This linkage is also shown in the relation between the
phenotype and the dose of Spra in the kernel.  ihe higher the dose
of a, the fewer and smaller the pigmented spots.   The
majority of kernels with only a few specks of pigment as in the
wx class.
-      kernels with many pigmented spots, some of which are

large are present in both classes but mainly in the tix class.
                                                                     _-