lor to try to answer tb qwrsltlona you rainail in yaar letter,  ft 840rpI to UB
that you me psrfaotly rete in rurumiag that tha arsayed aotivtty la a valid maiuro of
%ha mrrymatio oontent.  TbPt these aativities would be proportional  to tks rolatlto amount8
of sumtrate aotPsllr mtabolited would br tr\u honter only pgdor ths i0;ilowlag a8~mpp1
tlono: (1) She em- areaye4 la ths only metabollu pthway for mooiaefo. (2) Tou
are &eallw with an snayarptioally balrurared (aon-tranwformi~) cell,

To givo a trivial example whbh points up the moesrity of aaklrrg the reoond

aasumptlon we ma,y hagfir, galmtorpraare uro~rvr on a galwtom adaptad -11 no longer in
oontaot alth galaotom.  Under aertain ouw3ltlorrr 1 ~8n mairrtain the enegss alaoat  iadsfl-
nltely In the abreaoe of its substrate.  %hw oondltlanr ami erlat where non-f'unationlng
oar;ysma aan prrlrt In mearurablo sa~euntr and where mtitity n~mauresrsn$r bear no relatlms
to the mmunt of aubrttate motabolisml rla ths ar8ayed ersymo.  It la not unlikely however
that shoe you are Ueallng wlth tlorue u~algslr (5.0. oellt ahioh ham beea rvormd 8 long
ttm alpd have bad ample opportunity to get rid of non-funatlonal e~ymsr) you need not
oonoern grourrelf too nwrh about the nsoerrity of lnohhg eumnptlon (2) altho I think It
wuld be of valna to erplloitly state it,

lfhat would leave yoa Q aray out.

Aa to tbs data you request;

rme time ego I trlad to Obtain snoh data on

rwoinoriBa8e rlth one of my paut rtralnr.
form la the abseme of an external rouroe of nitrogen, Le., it ooald not, turllke otwr
era~g~er in thla strain, ao-te saooe8a~lly rlth the rxlstent enzyme8 for the oellnlar
sourm of protiena.
beeom8 ladspadeat of substrate level boyold 0.2 &  'phi8 I8 the uaml piatwe obtained
eal  io not mrprlainfg b view of the gelleral nature of onbatrat0 oonoentra~lon-rat8 ourvor.
The level o? aotlrity obtained ir a [t6=of 2.1 before Maptatfon and 48 after the ymthealr
1s ooaaplete.
ard 18 not dsrrotebly dlifemnt in the auoainate-adapted arrd non-adapted ae118.

I found tat thir gsrtfaulet en- would not

JPlth an external rmras of nitrogen the lent1 of ensyme wtirlty

YQU will bo laterestrd td note that thebal,aith respwt to gluf3ore is

90.3

1 8hWld like to Blspbuhe that &hi8 pfOt-6 18 trw only for 0- ertrain ud  I

don't know how general It lr a8 yet. Ye are going to get f'wthsr data on thlr polnt ainoe
raooinmidoae ir one of the e~llyl~sr releoted for our stud37 of aoznpetltlt. lntoraation dwfw
onrynr tormation.  ft lr of putloular interest for our program rlnae it represent8 001) of

Shorzlrl 9oa wiah to play armad with It youtrelf, don't heritate to do 80 and

if you waat 80- of em rtralas to do the job I aaald be Wpgp to send them Blong.

Your ooanarat re tb Zoo department, 'faas of boat rookina;' oOnf&rIm+2 q own

oonolurionr a8 to their probable repatiop.  Bltho I know suoh feara am groundless there
is unfortunately litt& I e8n do to diapel them.

By the way, I io- oat quite a bit arbout atle%lna.  PlthaPgh I 188 unaware of it

sad a. L.J. Stadler.  Both of thsa a88Wed IYI hs waa 8 flwr fellw who had a lot @I ability
sad would not llkaly bs one to %~y to organtoe aaybbdy out of fadepadent exi8ten~11.  !!e dM
a ifno Job at Ma0111 In building up 8 @enetio8 deprtment from soratoh.
go to 8ee his aa b, bprobably $rot mh to oontribute.  On the W818 of tha dlroprrio~ f
hod with Dr. Anderron I 8-11 8e88hia qr reprint8 leM? try to gst eoqusiatcrd ntyreli.

at

tihe tIP4 I h8t 8- YQU,

I; hPppba to LmOW tWO tery good

fFMrd8 Of hi8, Dro Ed- Bnderaon

I would by all mean8

     Thir letter is getting too long and so I will save the rest ?or P latef efr~rt.
I have 1-8 beautiful data now on ocapotitirr interootion between e,nqymer and most lropoctant
involving 8om oompoaentr of tta glueorymare ryatern whlah maker tk whole phenoaannon more
general and make8 me very happy.

S iaoeret J y airs,

s. Spfegglersrr

P.3.  Storell is aoued stif? that at the forthomi- mating you all1 .plmrise him with
   you? bioohslnioal erudition.  I triad to os15 hi8 fears (whioh apguently arom ti8
   a oorresgonbsnoe between you tro)  arb4 arsared him ym were (L rery gentle Sm%&x Doul
   rho didn't eat bob188 for brsakfart only energy rioh phosphate b-80