THE UNIVERSITY OF WISCONSIN

        THE MEDICAL SCHOOL

           MADISON S

MCARDLE MEMORIAL LABORATORY

FOR CANCER RESEARCH

3r . Ar tliur ICornS erg
Cxperiaental Biology and Xedicine Institute
2ationai Institutes of Eealth
3ethesda lL;, Ed.

3ear I&. Kornberg:

      I have your letter of lIay 5 regarding the preparation
of TKI. 3r. :,:uelier and I have been collaborating on this
project. :;le shall be very glad to help you bg letting you !inov:
of the procedure before it apTe3rs in yrint. As Jr. ?otter
evidently told you at the meetiqs, we had obtained pure TP;J by
a procedure which is much simpler than that of ,:arbur;, with
hi@i recoveries. It involves mi adsorption on activated char-
coal. ':;e were not inclined to give much information concerning
the procedure because we had some difficulty just before the
mectings. A new batch of charcoal arrived Miiicli did not behave
the same and we have since been working very hard to resolve
this difficulty. After a study of a nanber of charcoals, we
triink we have one which is reliable aid consistat in propeyt ies,
so that we are about to finish the project. It will be ?erha?s
two or three weeks before we have tine to check everytiiinz well
and ::et a r;laniiscript typed up. Then we shall be glad to send
you a copy.

In the xeantimc, in order to save you tiqe, I would

1il:e to md<e several suggestions concerning materials you will
need and could get ordered. I assu-le, since you are doins
st.-*ncture work, that you will want to make TPLT on a relatively
large scale. use 2ork liver ai-d have viorlieil un as inuch as
100 k:;. at once, froix which one gets approximately 3-4- ;.;rz:ns of
TPIi. The problera of IiaiiJlin~ the large vo1u:nes of extracts in-
volved here was solved by purchase of several 20 gallon gdvanized
ash cans which are obtainable in any iiarddare store for about
;2.:30 each. You need to purchase or arrange loan or an electric
driveii ixeat Zrintler, if you do not already possess one. Further,
I rrouli suzgest you pwc1,ase 5-10 lbs. each of two charcoals
knoun as "ldtlciiar C" aid "Xuchar Cl(30". These can be obtained at
tne IndGstr3ial Sales Xvisioiz of tile '.$est Virginia Pulp and ?aper
Company. I have dealt with their plant at Tyrone, Pa., but they
have offices at 230 Park Aveime, ;,e*,i Yo~k 17, LY.Y.

I wish you success witl; your structure studies.

Yours sincerely,