DEPARTMENT OF PHARMACOLOGY

NEW YORK UNIVERSITY

  COLLEGE OF MEDICINE!

(UNIVERSITY AND BELLEWE HOSPITAL MEDICAL COLLEGE)

477 FIRST AVENUE, NEW YORK 16, N. Y.

'ELEPHONE: ASHLAND 4- 1800

March 29, 1949

Dr. Arthur Kornberg
Experimental Biology and Medicine Institute
National Institutes of Hedth
Eethesda l.4, Maryland

Dear Arthur:

Please accept my best, if delayed, thanks for the sample
                   I an ~lso glad to have

We are about to make a batch of the 1st acetone precipit8.te

of TPM which arrived safely in due course.
the dete.ils of the counter current purification, it looks pretty
good.
and will try counter current on it.

There is a mschine in Chemistry.

     We got a copy of Rosenthal's acetoacetic acid method. Un-
fortunately, oxalacetic ret;c-ts as much es acetoacetic and, €or deter-
mination of the latter in the presence of the former, the method may
require a special study.  However, it will be quite useful for aceto-
acetic in the absence of interfering substmces and I am glad to have
it.

There has not been much progress recently in the citrate

synthesis.
from acetate 4- ATP + OAA.
erntion of ATP which disappeered Lfter rough fractionation with AmS04
and prolonged dialysis.
experiments with extracts indicating synthesis from the product of
the phosphoroclastic reaction can be interpreted in thet way.
we shall have to isolate (or try to isolate)  the "active" Lcetate fomed
both by the phosphoroclastic reaction and by acetate + ATP.
we should like to get evidence for the assumed formation of citryl phos-
phate.

1% found E. coli extrzcts very active in fo-rming citrate
      There seeoed to be"funny catdytic regen-
      In view of this, I ai not sure that earlier

I guess

Of course

'Ke sent a note to J.B.C. with the results on pigeon liver.

I VEG very pleesed that you founci good activity in pig liver

for the NXN -I- ATP--jDPid reaction and I an sure you will be able to
demonstrete the participation of pyro-P without too much trouble.

Dr. A. Kornberg

-2-

Xarch 29, 1949

I know exactly how you feel about sitting at a desk all day.

I am in the sane boat working on the article for Sumner & B;iyrb&kls
book.
gives you i! chance to write up your papers.

You should not be too unhappy a'mut it, however,  since that

We shall be seeing each other soon and I look forward to it.

Meanwhile my best to everybody in the lab with ow fonZest regards to
the Kornberg familjr.

so : ims

Severo Ochoa