DEPARTMENT OF PHARMACOLOGY

NEW YORK UNIVERSITY

         COLLEGE OF MEDICINE
(UNIVERSITY AND BELLEVUE HOSPITAL MEDICAL COLLEGE)
    477 FIRST AVENUE, NEW YORK 16, N. Y.

TELEPHONE: ASHLAND 4-1800

January 3, 1949

Dr. Arthur Korn'werg
Division of Physiology
National Institute of Health
Bethesda l4, Maryland

Dear Arthur:

     I have your letters of December 28 and 31 and I am writing
to Ohlmeyer along the lines you suggested in the first letter.
suggested. to him that he forget about the Lithuanian Physicist so as
not to create further complications.

I also

I am afraid we have used up a great deal of the Zmischenferment
       I will check on tha% and while I would like to keep

that Bernie gave me.
a couple of milligrams I will send back the remainder.

     I was glad to hear that you have gotten positive results With
the synthesis of F.A.D. and I think it is very interesting to follow it
up.  From what you say and what might be expected, presumably a similar

system is at mork as in the case of the D.P.N. synthesis.
to see your letter in the December issue of the J.B.C.

It was nice

We are certainly trying to get the biotin story on simpler
                      I have been thinking

ground# but this doesn't seem easy at present.
that if one could get a protein fraction with activity on the phosphory-
lation system one might perhaps make a start in that direction.
we had an unexpected finding, namely that biotin deficient cells of
L. arabinosus contain five to ten times as much D.P.N. 8s the bateria
grown in presence of enough biotin.
VES detsrmined fluorometrically by Huff an6 Perlxweig's method or enzy-
matically using alcohol dehydrogenase, flavurotein and dichlorophenol-
indophenol.  While this difference might to some extent be due to dif-
ferences in extractability it might also mean that biotin might be in-
volved in D.P.N. metabolism.  I wonder whether this vitamin may no&be
concerned with systems such as the ones you are investigating and,,on
the possibility that these system may indeed be related to aerobic
phosphorylation.  I will Let you kaow of any further progress we may
make and we mi-ght then discuss whether some common effort would be
profitable.

Eecently

This was true vhether the D.P.N.

Dr. 8. Kornberg -2- January 3, 1949

Before I close, let me thank you for the yeast which arrived in good
condition lzst Friday.

I am enclosing a copy of Grafflin's test for oxalosuccinic

carboxylase.

With our fond regards to Sylvia, the children and yourself.

SO : ims

P.S.

Uuch to my consternetion I find that theye are only a few mg.
of Zwischenfermmt left.

I am sending it back.