Human Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence
This article was the result of collaboration between various members of Anfinsen's lab at the NIAMD with Hunkapiller and
Hood at Caltech. In it, they addressed the difficult problem of determining the amino acid sequence of Human Lymphoblastoid
Interferon (HuIFN-alpha). Earlier attempts to sequence the amino-terminal portion of this molecule using conventional methods
failed due to insufficient quantities of interferon. Through this collaboration, Anfinsen's lab developed a microsequencer,
a technology that allowed them to obtain amino acid sequence data on 10 to 20 picomoles of protein. Because of this, they
were able to successfully determine the amino-terminal amino acid sequence of the major component of human lymphoblastoid
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Zoon, Kathryn C.
Smith, Mark E.
Bridgen, Pamela J.
Zur Nedden, Dorothy
Miller, Dorothea M.
Anfinsen, Christian B.
Periodical: Zoon, Kathryn C., Mark E. Smith, Pamela J. Bridgen, Dorothy Zur Nedden, Dorothea M. Miller, and Christian B. Anfinsen. "Human
Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence." Annals of the New York
Academy of Sciences 350, (1980): 390-398. Article. 9 Images.
New York Academy of Sciences
Reproduced with permission of the New York Academy of Sciences.