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The Joshua Lederberg Papers

Protoplasts and L-Type Growth of Escherichia coli Annotation pdf (2,972,434 Bytes) ocr (73,953 Bytes)
Protoplasts and L-Type Growth of Escherichia coli
Number of Image Pages:
18 (2,972,434 Bytes)
1958-02 (February 1958)
Lederberg, Joshua
St. Clair, Jacqueline
Periodical: Lederberg, Joshua, and Jacqueline St. Clair. "Protoplasts and L-Type Growth of Escherichia coli." Journal of Bacteriology 75, 2 (February 1958): 143-160. Article. 18 Images.
Lederberg UI: P70
American Society for Microbiology
Reproduced with permission of the American Society for Microbiology.
Medical Subject Headings (MeSH):
L Forms
Escherichia coli
Drug Resistance, Microbial
Cell Wall
Lederberg Grouping: Published Scientific Article
Box Number: 80
Folder Number: 42
Unique Identifier:
Accession Number:
Document Type:
Physical Condition:
Series: Writings
SubSeries: Published Scientific Articles
Folder: P70: "Protoplasts and L-Type Growth of Escherichia coli" (1958)
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Annotation by Joshua Lederberg:
KW: protoplasts; penicillin; L-forms; spheroplasts; Dienes;
Klieneberger-Nobel; DAP, diaminopimelic acid;

My interest in L-forms had several sources.  They had played a part in
pre-1946 imaginations of cellular changes in bacteria; even 
"zygospores" - Mellon - .. to the point that Louis Dienes had been
invited to one of the Cold Spring Harbor Symposia; and I had visited
Dienes in Boston.  Cf also Amsterdamskaya papers re cyclogeny.
Zinder & I had seen strange, filtrable forms in early interpretations of
transduction; I even expanded on that in CSH 1951.  That blew up of 
course and "FA" became phage.

Nevertheless, after reading Weibull and Zinder on formation of
protoplasts with lysozyme, and their stabilization with hypertonic
sucrose; it came to me that penicillin-lysis might be intermediated
by osmotically fragile wall-defective forms; and one Saturday afternoon
experiment substantiated that.  While this was the first rationally
based examination of these odd forms, there remain many mysteries --
the role of agar, permanent L-forms; mg++;  and what can be done
with them icw cell fusion.

L-forms also play some odd role in persistent, antibiotic-resistant
chronic infection esp. streptococci.

More: LOOKUP: exact interplay of Zinder, Weibull articles.;

Correction:  bibliographic reference to

WORK, E. 1957 Biochemistry of the bacterial
  cell wall. Nature, 179, l-16. -- should be pp.841-847
The 1-16 doubtless refers to pagination of a reprint I had;

DAP analogues have been explored as antimicrobials:  RJ Cox and

jl 12/15/98;  1/24/99;  8/20/02