As you can see from the letterhead, Esther and I are visiting California this summer, which accounts for the delay in my reply
to your letters which I have just received. Right now, in fact, we are at Mather, where Dobzhansky has set up his collecting
stations, and Stebbins is conducting a field trip in plant taxonomy and evolution. We are returning shortly to Berkeley,
at the above address.
Of your three proposals for elaborating on the linkage analysis, your first seems the soundest. I don't understand the
second "maps obtained when small amounts of the selected factors are present". The third (comparision of single with
odd n-ple crossover classes) would be very involved especially if interference (positive and negative) must be considered.
I am still convinced that you should publish your significant results. Newcombe just now sent an MS showing crossover data
among prototrophs from such crosses as W-677 Sr x 58-161, with and without further selection with streptomycin (to collect
the rare Sr prototrophs). He thinks he can simply map S, Mal, etc. to the left of M, and explain away additional discordancies
as "negative interference". I mentioned, briefly, your line of research, but suggested that he consult you for details.
You once mentioned that nutritionally complementary selections (not necessarily associated reciprocals) failed to show complementary
segregation for Mal. If this is verified, then any simple mapping with prototrophs becomes vitiated, at least for the present.
Newcombe also has not encountered the anamalous effect (or rather lack of it ) of B1 on these segregations which would appear
to necessitate placing the B1 locus on a separate, third branch from Mal, S, etc., on the one side and Lac, V1 on the other.
Negative interference does not seem to me to explain anything, and I suspect that a good many people might be more inclined
to reject the concept of linearity as a proven mechanism in E. coli, rather than to accept this modification. Your data would
really help to clear the air considerably, if they are extensive enough for you to be willing to publish them.
Before closing, may I refer to any part of your unpublished work in a resume of (part of) bacterial genetics to be used as
an address at the Columbus Genetics Society meetings. It might be best if you would mention specifically what approaches
and conclusions I may cite, if any. If possible I would like to have this within a month, although later revision (til 13)
is possible. Thanks very much.