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The Joshua Lederberg Papers

Title:
Letter from Mirko Beljanski to Joshua Lederberg Annotation pdf (141,691 Bytes) transcript of pdf
Letter from Mirko Beljanski to Joshua Lederberg
Description:
Item is handwritten.
Number of Image Pages:
2 (141,691 Bytes)
Date:
1956-10 (October 1956)
Creator:
Beljanski, Mirko
Recipient:
Lederberg, Joshua
Rights:
Reproduced with permission of Monique Beljanski.
Relation:
Lederberg Grouping: Correspondence C
Box Number: 9
Folder Number: 25
Unique Identifier:
BBACIY
Accession Number:
5
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Correspondence, 1935-2002
SubSeries: 1953-1960
Folder: Beljanski, Mirko
Transcript:
10/56
Dear Prof Lederberg,
Thank you for your letter of Sept 24. Some days ago I had a discussion with Dr. Ogiusici[?] about streptomycin-resistant mutants. I do not agree that all streptomycin-R mutants have a respiratory defect, because I had many mutants with normal respiratory enzymes. But it is possible to get from E. coli posphyrin-negative mutants in the presence of streptomycin.
At the beginning we have isolated from E. coli ML posphyrin-less mutant by transfer of the normal bacteria into liquid medium with increasing amounts of streptomycin sulfate. In this way, we had much difficulty to obtain it again. So we looked for a method to obtain regularly porphyrin-less mutants. As this mutant was sensitive to homin, we have combined the action of homin and streptomycin on the normal bacteria.
Techniques
medium: 20 gr peptone "Uclef"
2 gr glucose
5 gr Na Cl
1000 ml H20, ph 7.4
hermin solution: 5 mg hermin -> 3 ml Na2HPO4 buffer M/2. Heating at about 70-80 degrees C. After dissolution, complete the volume at 25ml(H20). Sterilisation [sic]10 min at 105 degrees C. Use always fresh solution (1 - 3 days) at 5 degrees C
Streptomycin sulfate has been used.
[END PAGE ONE]
[BEGIN PAGE TWO]
Essai[?] tubes containing 10 ml of peptone water
[IMAGE]
After 24 h, isoculum is taken in the tube with growth. So after some transfers into the next tubes we obtained a culture the optical density of which is more less than in the normal state. Transferred on solid medium, the mutant gives very small colonies.
The same mode (with mernia) of isolation but with solid medium (Petri dishes) was unsuccessful.
We have obtained the posphyrin-negative mutants from E. coli M.L. ; B ; K-12 (CGoo); but we could not get them from K-12(112)*.
We do not know the mechanism of the appearance of such mutants. This problem has not been studied.
My best regards
M. Beljanski
*K-12 strains from Dr. Wollman and Jacob
Metadata Last Modified Date:
2017-06-29
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Annotation by Joshua Lederberg:
KW: streptomycin-resistance and porphyrin negative; Schaeffer (?);

see papers in CRAS or CRSB?

[has this been corroborated?  catalase-deficient?]

I must have wondered if it was a plsmid cure.

jl 12/26/98