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The Joshua Lederberg Papers

Letter from C. E. Clifton to Joshua Lederberg Annotation pdf (119,071 Bytes) ocr (2,078 Bytes)
Letter from C. E. Clifton to Joshua Lederberg
Number of Image Pages:
2 (119,071 Bytes)
1950-10-05 (October 5, 1950)
Clifton, C. E.
Stanford University. Department of Bacteriology and Experimental Pathology
Lederberg, Joshua
Reproduced with permission of Stanford University.
Lederberg Grouping: Correspondence B
Metadata Record Letter from Joshua Lederberg to C. E. Clifton (September 29, 1950) pdf (56,759 Bytes) ocr (643 Bytes)
Box Number: 6
Folder Number: 180
Unique Identifier:
Accession Number:
Document Type:
Letters (correspondence)
Physical Condition:
Series: Correspondence, 1935-2002
SubSeries: 1947-1953
Folder: Clifton, C. E.
Metadata Last Modified Date:
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Annotation by Joshua Lederberg:
KW:  E. coli K-12 origin

Clifton describes its original isolation in 1922 as a typical
E. coli lab strain.  responds to my query BBAAEH 9/29/50

jl 8/20/98;  5/7/05

STANFORD UNIVERSITY STANFORD. CALIFORNIA DEPARTMENT OF dACTERIOLOGY AND EXPERIMENTAL PATHOLOGY October 5, 1950 Dr. Joshua Lederberg Department of Genetics University of Wisconsin Madison 6, Wisconsin Dear Dr. Lederberg: I am sorry to have missed you during your visit on the Stanford Campus this summer. I have followed your studfes with great interest and hope that you will place my name on your mailing list for reprints, I have placed your name on my list as requested. Escherichia coli (K-12) was isolated by Dr. Blair in the fall71922 from the stools of a diphtheria convalescent and was identified at that time as an E. coli by the ordinary laboratory tests, It has been maintained in our stock culture collection since 1925 and is used in our laboratory as the typical coli culture. Many of our studies on bacteriophagy and on growth and metabolism have been carried out with this strain, cultures of which we have supplied other laboratories and individuals, We have recorded the following characteristics of this strain: Morphology and staining reactions : Small plump gram negative rods with rounded ends occurring singly. Motile l  Characteristics on solid media: smooth whitish, moist growth, E.M.B. agar: colonies show metallic lustre. Potato: yellow-brown growth. Simmonts citrate agar: no growth. NacConkeys : good brick red colonies, Endo: good - excellent metallic sheen, Endo + l/2$ lithium chloride: not as good as Endo. E.M.B.: good - colonies small - metallic sheen. Characteristics on liquid media: Plain broth: turbid, slight pellicle and sediment. Dr. Joshua Lederberg Page Two October 5, 1950 Biochemical activities: V.P. -. M,R, -t-, Lead acetate -, Xitrates reduced. Indol +. Gelatin not liquefied, Litmus milk acid and coagulation. Acid and gas in dextrose, lactose, mannite, maltose, salicin, dextrin (~1,) galactose, levulose, glycerol and dulcite. No action in sucrose. Sincerely you s )@j!!lqL C. E, Clifton CEC:es