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The Joshua Lederberg Papers

Letter from Michael Doudoroff to Joshua Lederberg Annotation pdf (181,021 Bytes) transcript of pdf
Letter from Michael Doudoroff to Joshua Lederberg
Mike Doudoroff
Item is handwritten.
Number of Image Pages:
2 (181,021 Bytes)
1948-07 (July 1948)
Doudoroff, Michael
Lederberg, Joshua
Reproduced with permission of Michael John Doudoroff.
Lederberg Grouping: Correspondence B
Box Number: 6
Folder Number: 216
Unique Identifier:
Accession Number:
Document Type:
Letters (correspondence)
Physical Condition:
Series: Correspondence, 1935-2002
SubSeries: 1947-1953
Folder: Doudoroff, Michael
Dear Lederberg,
I am writing you from the Sierras, where my wife and I are camping together until the Hassids.
I have been working with your maltose and glucose-strain of E. coli and we've got some extremely interesting results. As soon as I return to Berkeley, we shall attack the problem with everything at our own disposal.
To summarize the results to date briefly;
(1) Your analysis was quite correct. Both the fermentation and the recuperation of maltose are much more rapid than of glucose.
(2) Dry cell preparations also ferment maltose but not glucose.
(3) In the presence of poisons, phosphate is esterified, indicating a phosphorolytic mechanism.
(4) The analysis is greatly complicated by the simultaneous breakdown of reserve materials (apparently glycogen) in the bacterial cells.
If we can prove phosphoroulysis of maltose, we shall want to report it as soon as possible,
and since, naturally, we shall do so with you as co-author, we should like a few more details about the culture, etc. as follows.
(1) The exact nomenclature of the strain and its character.
(2) The distribution of the strain and the successive mutations involved
(3) Any financial support of your work that you wish mentioned in the footnotes.
Needless to say, we shall send any forthcoming manuscripts first to you for approval.
In addition to the above information, I would like to have the following;
(1) The wild type strain to compare with the mutant.
(2) Any other interesting strains, such as
(a) trchalose positive glucose negative
(b) lactose positive, glucose and galactose negative
(c) " " galactose negative
(d) melibrose " glucose and galactose negative
(e) " " galactose negative
If I can get a fellowship for my affiliated year (1949-50) I would like to spend part of the year with you trying to pin down some of the genes you are working with the particular enzymes. I am extremely encouraged by the results to date and find that E. coli is a very good bug to work with. Please excuse this hasty and semi-literate letter.
With warmest regards,
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Annotation by Joshua Lederberg:
KW: P-18  ; maltose metabolism;

starting collaboration on Mal+ Glu- mutants, eventually led
to discovery of the amylomaltase pathway;

jl 11/7/98