I wonder whether it would be possible for me to obtain strains of K12. I would like to use them to attempt to prepare mutants
by the penicillin[?] method--mutants connected with [...] synthesis.
I would rather start with a sexual organism if possible and that is why I have thought of your K12 strain. I am told however
that there's the added complication of prophage. Do you have any K12 free of prophage? Or does that make any difference
For [...] mutants it would be ideal to start with a highly aerobic, rapidly respiring bacterium--on the assumption that the
more actively respiring ones have more [...] enzymes. Does your K12 use up lots of O2 under aerobic conditions? Could one
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penicillin technique on some other actively aerobic bacterium--other than B. coli?
I shall be gratified for any advice and suggestions you may have.