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If you agree with me on the value (?) of the MS I sent to you I think that the next thing, for me, to do is to look into the
fact that lambda HFT (from the 112 Gal- transduced cell) does not multiply in a GaI- cell. I would like to prove that lambda
HFT multiples in a Gal+ cell and does not multiply as HFT in Gal- cell, not picking[?] the Gal- marker of the host cell (I
have prelimaniry [sic] experiments with a bad Gal- of Bollman[?] that seems to show that) or picking it and becoming a new
For these experiments I would need then your Gal1- and Gal2- strains sensition[?] to lambda (I think the other nutritional
markers dont [sic] matter unless you tell me why) and just in case Gal1- and Gal2-. If I need to make them lysogenic I would
prefer to have markers (+++) (according to Dale Rainer[?]) on the prophages.
So I would be very grateful to you if you could send me these three strains in Pasadena where I expect to be back in a week
Thanks in advance
P.S. Arbor tells me that after transduction with lambda HFT inactivated by UV most (if not all) of the transduced cells are
stable Gal+ (and thus not heterogenates). Did Morse find the same thing?