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The Joshua Lederberg Papers

Title:
Letter from P. R. Edwards to Joshua Lederberg pdf (132,128 Bytes) transcript of pdf
Letter from P. R. Edwards to Joshua Lederberg
Description:
Item is handwritten.
Number of Image Pages:
1 (132,128 Bytes)
Date:
1958-09 (September 1958)
Creator:
Edwards, P. R.
Recipient:
Lederberg, Joshua
Rights:
This item is in the public domain. It may be used without permission.
Relation:
Lederberg Grouping: Correspondence C
Box Number: 10
Folder Number: 25
Unique Identifier:
BBAIFH
Accession Number:
13
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Correspondence, 1935-2002
SubSeries: 1953-1960
Folder: Edwards, P. R.
Transcript:
Dear Joshua -- I'm confused and appealing for help. It seems to me that the incidence of successful transductions in our lab is much lower than in yours. This week I used PLT22 phage of a liter of ca. 10 to the 10 in trying to transduce motility to a nonmotile S. paratyphi B which is susceptible to the phage. I made 10 tubes using a total of 4 ml of lysate -- not a single bulb appeared.
I am enclosing a reprint which gives our exact method for your convenience (p 280). Discard it when you have finished. Any comments would be appreciated.
Should we use fewer bugs?
How much of the phage-recipient mixture do you put on one if you've small plates? [*]
Should we eliminate the incubation for phage absorption? etc etc etc
If you could answer soon it would help I have a lot of non-motiles on the fire
Kindest regards to you and Esther
As ever
Phil
[*]How many plates per transduction?
Metadata Last Modified Date:
2011-08-08
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