Dear Joshua -- I'm confused and appealing for help. It seems to me that the incidence of successful transductions in
our lab is much lower than in yours. This week I used PLT22 phage of a liter of ca. 10 to the 10 in trying to transduce motility
to a nonmotile S. paratyphi B which is susceptible to the phage. I made 10 tubes using a total of 4 ml of lysate -- not a
single bulb appeared.
I am enclosing a reprint which gives our exact method for your convenience (p 280). Discard it when you have finished. Any
comments would be appreciated.
Should we use fewer bugs?
How much of the phage-recipient mixture do you put on one if you've small plates? [*]
Should we eliminate the incubation for phage absorption? etc etc etc
If you could answer soon it would help I have a lot of non-motiles on the fire