Thank you for your letter. I am very glad you agree about the Royamont project and am looking forward to seeing you again
We have not yet any precise opinions about the F factor since this does not seem apt to be any conclusive evidence its cytoplasmic
vs. nuclear nature (at least not yet we know of).
About the questions you ask, I can only answer some of them.
1). To plate [. . .] one has to be very tired[?] with the [. . .] couples. We dilute very slowly, throw gently [. . .] into
melted agar tubes and pass these onto agar plates, very carefully.
2) We have no more CSH reprints. I will look carefully whether some have been hidden somewhere and send them if I can find
3) We have some [. . .] for which the first [. . .] enters between 4 and 5 [ . . . ]. This is the earliest time[?] we have.
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4) We have not yet studied zygotic [. . .] very early.
We have another evidence that in some cases the whole genome[?] is infected: this is based on [. . .] B galactosidase by zygote
resulting from [. . .] between H lac+ and F- lac-, in conditions where the [. . .]. If our uses are off strain which injects
lac in the first minute, once [. . .] that B-galactosidase [. . .] almost immediately after the [. . .] of the first lac [.
. .] in the recipients and the [. . .] where [. . .] lac [. . .] are transferred (all this is independent of 2c [. . .]).
When one uses all [. . .] in which lac is ejected last, there is also detectable [. . .] of [. . .] for several hours.
We shall discuss all this next summer. I hope you [. . .] enjoy the Royamont meeting which we shall try to be pleasant, not
too tiring, and if possible useful.
Best regards to both of you and to Luca
I just realize that the secretary did not possess[?] the paper I had prepared to send with the [. . .] for Richter. Would
you tell him that [. . .] is B- and lac-.