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The Joshua Lederberg Papers

Letter from Charles Yanofsky to Joshua Lederberg pdf (104,266 Bytes) transcript of pdf
Letter from Charles Yanofsky to Joshua Lederberg
Item is handwritten.
Number of Image Pages:
2 (104,266 Bytes)
1958-05-29 (May 29, 1958)
Yanofsky, Charles
Lederberg, Joshua
Reproduced with permission of Charles Yanofsky.
Lederberg Grouping: Correspondence C
Box Number: 14
Folder Number: 189
Unique Identifier:
Accession Number:
Document Type:
Letters (correspondence)
Physical Condition:
Series: Correspondence, 1935-2002
SubSeries: 1953-1960
Folder: Yanofsky, Charles
[written in Lederberg's handwriting, Yanofsky]
May 29 1958
Dear Josh,
I'm enclosing 9 stocks which have not been messed-up yet and two which are in a different category, as you will see!
T-3 [unknown symbol] ansh (h k-12 by U.V.)
td4 I [unknown symbol] T "
T-4 AA [unknown symbol] I "
Hist minus cys4 reisolate (k-12) from Treffers [?] -- a fairly good stock if grown in the presence of cysteine supplement (30 gamma/ml) -- all In rich media and slants
"Hfr" H minus T-3 -- transduced "B" tryp region (with requires) into H minus cysq and isolated an H plus with requires -- replicated for an Hfr (1) and got one -- replaced tryp region by transduction from H minus cys4 -- picked a H minus cys minus -- replaced cys with T-3 by transduction
undoubtably lysogenic for Plke --
This stock and other isolated from Hfr(1) gives 5.5 percent recomb. when crossed to strain B F minus's and to avalent K-12's. We only get high frequency when we plate 1 hr old mixture on enriched minimal (.2 percent by volume, nutrient broth soln supplement) and if we include a supplement permitten, recovery of the non-hist marker of the donor ([ . . . ] A. with HfrH minus T-3)
Recomb. colonies tend to be se[ . . . ]ed. Really don't know what grew with this strain, would appreciate your opinion if you can run a cross with it.
Hfr H minus/1tryp number delta -- mostly organic for Plkc from Hfr (1) by exposure to F1. Isolated typical tryp deletion type -- responds to tryparue and is V1R. V1 is of course very closely linked to the tryp region. We have been able to transducer the tryp deletion into a cys attach.
8am order a basis of crosses and transduction.
If any of these prove useful let me know and I'll send you a copy of my stock list -- we have many combinations which you might find useful. --
I'd like a transfer of the gal stock you think is closely linked -- it might be close enough for our transduction mapping.
Hope Elway will give you what you want -- this place will probably continue to be almost unbearable if it remains as is --
Best regards,
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