On the enclosed sheet are a few series of shots taken with oil immersion, Micro-file film, low intensity of light, 4 second
exposure. The prints are sloppy and unenlarged, but you can get the general idea from them. The cells sprouted so fast during
certain periods that critical pictures are missing. Will try to focus attention upon fewer cells and telescope observations.
A few of them are 400x and labeled High Dry.
Spent -- rather wasted -- a couple of days trying to follow cells that progressed no farther than number 1 on other sheet.
Then theorized that perhaps combination of brighter light via oil condenser plus concentration of rays by immersion oil produced
too much heat (or light???), because preps prepared at same time and with same ingredients grew well either when left alone
or observed with high dry. Longer exposure with less light (field poorly visible to eye) allows normal growth and also gives
I believe that these pictures, at least when printed properly, are about as well as we can do with 35 mm setup. Perhaps we
would not have to sacrifice depth of focus by using plates or larger film with 400x scope magnification.
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[BEGIN PAGE TWO]
Ran a large number of platings of Y10 with 50 units of penicillin, and after 6 days of incubation there are a few suspicious
looking colonies, but I refuse to become excited till the acid test. Also ran some reconstruction plates containing 1:1000
DAP minus; only a small percent came through (assuming fully viable initial population), but they are detectable. New crosses
are being run. Bessie stopped in last Friday and said the electron micrograph specimens look fine -- plan to devote this week
News Briefs: Alan won NSF Fellowship. Wolfram's daughter has measles. No news (that I know of) from Orskovs. 3" of
snow so far today. Lab running smoothly.
P.S. Hi, Esther.
Thanks for cards. We all envy you, especially at the moment, with the sudden reappearance of Jack Frost.