In support of my application for laboratory priveleges [sic], I should like to bring to your attention the following program
for work in histology and cytology.
1. Cytology and the Theory of Fixation of Plant and Animal Cells.
a. Sections of onion root-tip, fixed in Fleming's or potassium dichromate, -chromic and, -acetic and fixative, stained
with non-hematoxylin [?] or gentian violet, for study of the structures both the chromatic and achromatic figures, and as
a reference for the following:
b. Sections of onion root-tip, fixed in Baum-Allen's B15 fixative adjusted to various pH's, to determine effect of
fixative pH on fixative results. Correlated with this, each member of the series is to be stained with non-hematoxylin [?],
gentian violet, methyline blue, Delafield's hematoxylin, acid fuchsin, and picro-carmine to discover the optimum stain
at each pH.
c. Sections of frog testis, same as above.
d. If the above is ever completed, there are a number of other fixatives whose effect varies with their pH. However, a definite
conclusion might be derived from the above.
e. If osmic acid is available, the interactions of the concentration of osmic acid and time of exposure in fixatives in determining
over-fixation or osmication of cells.
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f. Inasmuch as osmic acid is so expensive, an investigation to find cheaper satisfactory substitutes would be fruitful in
making more facile the work of all cytologists.
g. A comparison of the osmic acid and ferrous ion techniques in detection of the Golgi bodies and a comprehensive review of
the literature on them as regards their existence in the living cell, and the formulation of my own criteria.
h. Determination of mitochondria by other than osmic fixatives and stain procedures
i. In vitro reactions of and production of artifacts by osmic acid.
j. Relation between differential staining and particle s[ . . . ] in emulsions of albumen
2. Histology -- The comparative Histology of the vertebrate Stomach.
a. Serial sections, of intervals of 100 microns, of some typical mammal's stomach as a reference series. Stain (Ion-)hematoxylin-resin.
b. The same for the stomachs of other representative vertebrates -- Turtle -- Reptilia, Mouse -- Mammalia, Frog -- Amphibia,
Sparrow -- Anco, Perch -- Pisces, and others if available. From these cross-sections, the construction of detailed models
relative to the distribution of the finders [?] pyloric and cardiac glands and the thicknesses of various layers in different
regions, with a correlation to function in nutrition as far as possible.
I have had some experience in bio- and organic chemistry, especially the latter, and may on occasions, be called upon to make
my own stains in exceptional cases. However, the program outlined above is sufficiently exhaustive of prospective activity
in the happy event that my application, herewith enclosed, is accepted.
Very Sincerely yours,
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Current scientific or research activities:
Currently, I have been able to do work in histology. At the present time, I have been working on the root-tips of narcissus,
for mitotic figures, but inasmuch as I have not yet been able to section the blocks prepared, nor to stain any such sections,
I cannot say anything conclusive concerning my results here. For the past year or so I have been able, on a rather limited
scale to conduct a group in histology as part of the biology club. This group is engaged in studying slides that I have prepared
with their assistance. Currently, we have just, very cursorily, completed the stomach and the duodenum from their histological
aspects. In this way, I have been able to learn the technique. Therefore, I have prepared such tissues as I could get and
had time to handle, and have been unable to fulfill of organized integrated study. I am, however, interested in the comparative
histology of mammalian stomachs, with particular reference to the distribution and morphology, and function, of the glands
of the mucosa. As far as sum results are concerned, these can be put as answers to a number of individual problems -- "What
is the histological structure of such and such a tissue, and what is the most satisfactory technique in determining this."
In answer, I have slides of about 20 different tissues, some better than others -- rat embryo, frog marrow and blood, the
spinal cord of the frog, a 6-day chick embryo, head of a late snake embryo, frog stomach -- finders [?], and stomach -- pyloric,
pancreas, duodenum, Human, now frog, turtle, rabbit blood with various stains, cerebrum and cerebellum of cat, frog head,
frog tongue -- gold preparation, frog kidney, spleen and liver, ovident, the subcutaneous areolae connective tissue of the
rabbit, as a diversion, an earthworm, and a few inferior ones. The irregularity of the work, made necessary by departmental
demands upon the laboratory and its equipment, have, unfortunately, prevented a systematic plan of work. There are now, in
particular, a number of problems in cytology that I am most fascinated in, but have been unable to work on in school. It is
these problems -- relation of fixative to artifacts, and apparent cell-structure, and simple means of observing and preparing
such things as the "Golgi bodies," mitochondria, etc. I may be able to construct a crude micro-dissection apparatus
in one of the school shops, and could hope for nothing more than to apply it to the living cell if it is constructed. The
equipment needed is expensive, but widely useful and standard -- a three-objective microscope with Abbe Condmeu and immersion
objective, a rotary microtome, if possible -- about 125 dollars for the best models, (60-95 dollars or more -- at first hand)
slides, coverslips, staining jars, and various stains and fixatives, alcohol, dioxan [sic], xychal, and balsain, comparatively
inexpensive, and some of which I can provide myself.