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The Oswald T. Avery Collection

Title:
Letter from Joshua Lederberg to Oswald T. Avery pdf (101,718 Bytes) transcript of pdf
Letter from Joshua Lederberg to Oswald T. Avery
Description:
In this letter, Lederberg asked if Avery could share a sample of p-nitrophenol-b-galactopyramoside from a study Avery conducted with Walther Goebel in 1929.
Item is a photocopy.
Number of Image Pages:
2 (101,718 Bytes)
Date:
1948-01-15 (January 15, 1948)
Creator:
Lederberg, Joshua
Recipient:
Avery, Oswald T.
Rights:
This item is in the public domain. It may be used without permission.
Subject:
Medical Subject Headings (MeSH):
Enzymes
Equipment and Supplies
Exhibit Category:
The "Sugar-Coated Microbe" and the Search for a Cure for Pneumonia, 1919-1929
Relation:
Metadata Record Letter from Oswald T. Avery to Joshua Lederberg (January 20, 1948) pdf (42,357 Bytes) transcript of pdf
/ps/access/CCAAFS.pdf
Box Number: 1
Folder Number: 2
Unique Identifier:
CCAAFR
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Personal and Biographical, 1931-2000
Folder: Additional Materials Relating to O.T. Avery, 1931-1948, 1960-1972, 1987-1999
Transcript:
January 15, 1948
Dear Dr. Avery,
Since my establishment here at Wisconsin, I have been occupying myself with applying the genetical technique Tatum and I have learned with E. coli to the problem of gene-enzyme relationships in this bacterium. To that end, I have been studying mutations affecting lactase activity, and it may suffice to mention now that a plurality of them have been found, somewhat to my surprise. I would have predicted that all mutations affecting a single enzyme would involve the same gene. The problem: is there indeed but a single enzyme?
I am addressing this letter to you to enquire whether you still happen to have a sample of p-nitrophenol-b-galactopyramoside, reported by Goebel and yourself nearly two decades ago (JEM 50, 1929). It occurred to me that this compound, in view of the pH shift in the absorption spectrum of p-nitrophenol, might be a useful chromogenic substrate to assay galactosidase activity. If possible, I would like to test this notion on a small sample (1-2 gms.) before undertaking a large scale preparation. While I would like to ask for this quantity, I could run tests on, say, a tenth that amount. If you can find the bottle, your cooperation would be greatly appreciated.
Best regards,
Sincerely,
Joshua Lederberg
Assistant Professor of Genetics
Metadata Last Modified Date:
2008-06-13
Linked Data:
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