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The Paul Berg Papers

Title:
Letter from Francis Crick to Paul Berg pdf (55,775 Bytes) transcript of pdf
Letter from Francis Crick to Paul Berg
Number of Image Pages:
1 (55,775 Bytes)
Date:
1973-12-21 (December 21, 1973)
Creator:
Crick, Francis
MRC Laboratory of Molecular Biology
Recipient:
Berg, Paul
Source:
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Paul Berg Papers
Rights:
Reproduced with permission of Gabrielle Crick.
Subject:
Medical Subject Headings (MeSH):
RNA
DNA
Exhibit Category:
Protein Synthesis, Tumor Viruses, and Recombinant DNA, 1959-1975
Relation:
Metadata Record Letter from Paul Berg to Francis Crick (November 14, 1973) pdf (60,124 Bytes) transcript of pdf
/ps/access/CDBBBC.pdf
Box Number:
3
Unique Identifier:
CDBBBB
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
21st December, 1973
Dear Paul,
Jack Griffith visited us yesterday and we had a thorough discussion about the SV40 mini-chromosomes. We learnt quite a bit from him about how he prepares them for the electron microscope and how he does the shadowing. Apart from the things I mentioned to you in one of my previous letters, we suggested that he had a try at selectively removing the two slightly lysine-rich histones, using Georgiev's method. This is done with transfer RNA. The hope is that what will be left, that is, the arginine-rich histones, will form a string of beads, rather like the picture you sent us that he got after a number of salt treatments. The thing we would like to know is, how many beads are there on the string when it is completely full of beads? We would hope the number was somewhere about 25. This might be quite an easy experiment to do and the result would be extremely useful in working out the numerology of chromatin.
Roger is working away like mad and has got an interesting X-ray pattern from the arginine-rich histones alone binding with DNA. Markus Noll now has an active preparation of the Hewish-Burgoyne enzyme, and a good assay for it, and is just about to start on the more interesting experiments.
Will you be over in the Summer? If you do come to Europe, we hope very much that you will drop in and see us.
Our best wishes to you all for the New Year.
Yours ever,
Francis
Metadata Last Modified Date:
2008-05-19
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