Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Paul Berg Papers
Reproduced with permission of Paul Berg.
Medical Subject Headings (MeSH):
Protein Synthesis, Tumor Viruses, and Recombinant DNA, 1959-1975
January 24, 1974
You may recall that some five years ago we had the cockeyed notion to use restriction enzymes to cut tumor virus DNAs at specific
locations. At that time you sent me some purified K restriction enzyme to test and as luck would have it, we chose polyoma
DNA to test and the K enzyme to restrict. That negative result turned us away from that approach and we've been kicking
ourselves ever since.
But now we have another use for the K enzyme and I'd like to impose on your generosity again if you have any of the purified
enzyme. As you may know, K enzyme does not cut SV40. We want, therefore, to use the enzyme to treat a mixture of RI cut
SV40 and cellular DNA in the hopes of reducing the fraction of DNA's which can form intramolecular circles by hybridization
and ligasing. Would it be possible to beg some from you or if you don't have any, to tell me who might have some? I'd
appreciate it greatly.
I haven't seen you for ages but I understand you've been very busy on the Vietnam defoliation project as well as the
many other things you manage to do. I gather there's a chance you'll be attending that Paris symposium on Man and
His Future in September. Hope to see you there if not sooner.