Skip to main contentU.S. National Library of MedicineU.S. National Library of Medicine

Profiles in Science
Pinterest badge Follow Profiles in Science on Pinterest!

The Paul Berg Papers

Letter from Paul Berg to Francis Crick pdf (71,866 Bytes) transcript of pdf
Letter from Paul Berg to Francis Crick
Number of Image Pages:
1 (71,866 Bytes)
1958-10-07 (October 7, 1958)
Berg, Paul
Crick, Francis
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Paul Berg Papers
Reproduced with permission of Paul Berg.
Medical Subject Headings (MeSH):
Exhibit Category:
From Classical Biochemistry to Molecular Biology, 1952-1959
Box Number:
Unique Identifier:
Document Type:
Letters (correspondence)
Physical Condition:
October 7, 1956
Dear Francis,
I'm sorry it took me so long to answer your letter but our baby was born this week and the resulting confusion has muddled things up somewhat. In any case I have enclosed a copy of the procedure we've used to isolate the acceptor RNA from coli. The only thing I should warn you about is that we usually do not carry out the heating in sodium lauryl sulfate on any larger scale than described although all other steps have been scaled up many-fold. If there are any difficulties don't hesitate to write.
Things have been going fairly slowly since we talked but we have succeeded in preparing four of the coli activating enzymes (leucine, valine, isoleucine and methane) free of each other and have been able to work out the detailed kinetics of the activation and transfer step. We have also, I think conclusive evidence that the coli RNA like the rat liver RNA accepts amino acids on the terminal nucleoside end and that there are separate chains for each amino acid. Moreover, we recently have been able to prepare chemically RNA preparations which will accept only a single amino acid but we have not yet achieved a complete physical separation of a homogeneous polynucleotide chain specific for a single amino acid. This is certainly the goal if we are ever to learn anything about what directs a given amino acid to a specific chain.
All of us here would look forward to your visit to our labs. We will probably move in June 1959 so if you are in the midwest before then we certainly would like to have you visit in St. Louis. If you do travel during the summer you can help us christen the new labs.
With best regards,
Paul Berg
Metadata Last Modified Date:
Linked Data:
RDF/XML     JSON     JSON-LD     N3/Turtle     N-Triples