Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Paul Berg Papers
Reproduced with permission of Paul Berg.
Medical Subject Headings (MeSH):
Protein Synthesis, Tumor Viruses, and Recombinant DNA, 1959-1975
Letter from Julian B. Fleischman to Paul Berg (November 23, 1962)
November 27, 1962
I don't know why you should think that measuring DNA polymerase activity in crude coli extracts would be any easier following
the incorporation of a ribonucleotide than in the more conventional way with four deoxynucleoside triphosphates. Doing it
by ribonucleotide incorporation is apt to give high values since you might also measure the activity of RNA polymerase and
the enzyme adding nucleotides onto acceptor RNA. As I gather it from the DNA polymerase people, measuring deoxynucleotide
incorporation in crude extracts is reproducible and reasonably accurate. The conditions for the assay are described by Lehman
et al. (JBC 233, 163, 1968). If your suggestion is based on the availability of labeled ribonucleoside triphoshates, I think
you would be better off making some dCTP32 or dTTP32 and using that in a straightforward DNA polymerase assay. For the preparation
of either of these you can use the chemical procedure described in the enclosed sheet, just substituting the appropriate deoxynucleoside.
Tell Mel that I'll write to him soon - I've been very busy trying to get my graduate courses for next quarter in shape.
I'm enclosing also a copy of the paper I gave at Rutgers which you can pass around or distribute copies of for whatever
Esther came back raving about Paris and especially you as a guide "housemate". There's something about Paris
that does wonders for everybody, since Esther just said "In spite of Palo Alto, I still miss Paris".