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The Paul Berg Papers

Title:
Letter from Paul Berg to Julian B. Fleischman pdf (65,907 Bytes) transcript of pdf
Letter from Paul Berg to Julian B. Fleischman
Number of Image Pages:
1 (65,907 Bytes)
Date:
1962-11-27 (November 27, 1962)
Creator:
Berg, Paul
Recipient:
Fleischman, Julian B.
Institut Pasteur (France)
Source:
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Paul Berg Papers
Rights:
Reproduced with permission of Paul Berg.
Subject:
Medical Subject Headings (MeSH):
RNA
DNA
Exhibit Category:
Protein Synthesis, Tumor Viruses, and Recombinant DNA, 1959-1975
Relation:
Metadata Record Letter from Julian B. Fleischman to Paul Berg (November 23, 1962) pdf (63,877 Bytes) transcript of pdf
/ps/access/CDBBDL.pdf
Box Number:
1
Unique Identifier:
CDBBDK
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
November 27, 1962
Dear Julian,
I don't know why you should think that measuring DNA polymerase activity in crude coli extracts would be any easier following the incorporation of a ribonucleotide than in the more conventional way with four deoxynucleoside triphosphates. Doing it by ribonucleotide incorporation is apt to give high values since you might also measure the activity of RNA polymerase and the enzyme adding nucleotides onto acceptor RNA. As I gather it from the DNA polymerase people, measuring deoxynucleotide incorporation in crude extracts is reproducible and reasonably accurate. The conditions for the assay are described by Lehman et al. (JBC 233, 163, 1968). If your suggestion is based on the availability of labeled ribonucleoside triphoshates, I think you would be better off making some dCTP32 or dTTP32 and using that in a straightforward DNA polymerase assay. For the preparation of either of these you can use the chemical procedure described in the enclosed sheet, just substituting the appropriate deoxynucleoside.
Tell Mel that I'll write to him soon - I've been very busy trying to get my graduate courses for next quarter in shape. I'm enclosing also a copy of the paper I gave at Rutgers which you can pass around or distribute copies of for whatever it's worth.
Esther came back raving about Paris and especially you as a guide "housemate". There's something about Paris that does wonders for everybody, since Esther just said "In spite of Palo Alto, I still miss Paris".
With best regards to all at the Institute.
Sincerely,
Paul Berg
Metadata Last Modified Date:
2008-04-30
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