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The Paul Berg Papers

Title:
Letter from Julian B. Fleischman to Paul Berg pdf (63,877 Bytes) transcript of pdf
Letter from Julian B. Fleischman to Paul Berg
Number of Image Pages:
1 (63,877 Bytes)
Date:
1962-11-23 (November 23, 1962)
Creator:
Fleischman, Julian B.
Institut Pasteur (France)
Recipient:
Berg, Paul
Source:
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Paul Berg Papers
Rights:
Reproduced with permission of Julian B. Fleischman.
Subject:
Medical Subject Headings (MeSH):
DNA
Exhibit Category:
Protein Synthesis, Tumor Viruses, and Recombinant DNA, 1959-1975
Relation:
Metadata Record Letter from Paul Berg to Julian B. Fleischman (November 27, 1962) pdf (65,907 Bytes) transcript of pdf
/ps/access/CDBBDK.pdf
Box Number:
1
Unique Identifier:
CDBBDL
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
23 novembre 1962
Dear Paul,
Mel mentioned that you have a method of assaying DNA polymerase activity in crude coli extracts by measuring incorporation of a labeled ribonucleoside triphosphate in the presence of three other deoxyribonucleotide triphosphates. If this is feasible, I would be most grateful if you would send me the details. Jean-Marie has prepared some labelled ribonucleoside triphosphates which could be used as substrates.
We are trying to screen a large number of temperature-sensitive mutant strains (i.e. strains which won't grow at 42 degrees C) for possible inactivation of DNA polymerase at this temperature. We have selected a number which, on the basis of thymidine and P32 incorporation, seem to be unable to synthesize DNA at 42 degrees. We would therefore like to set up a convenient in vitro assay for polymerase activity, preferably in crude extracts, so details of your method, or any other suggestions, would be most welcome.
Es has probably reported all the activity going on here at present. The only change is that the weather has turned thoroughly lousy. In spite of Paris, I still miss Palo Alto.
Best regards,
for Julian B. FLEISCHMAN
The Secretary
Metadata Last Modified Date:
2008-05-28
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