Quantitative Studies on Antibody Purification: I. The Dissociation of Precipitates Formed by Pneumococcus Specific Polysaccharides
and Homologous Antibodies
Throughout the early 1930s, Heidelberger, Forrest Kendall, and Elvin Kabat continued their work to elucidate the exact chemical
structure of antibodies, and namely to determine whether antibodies were modified serum globulins (a class of proteins in
the clear liquid portion of the blood that separates out upon coagulation). In this article, Heidelberger and Kendall detail
their technique for isolating pure antibodies by using varying concentrations and types of salts to break apart the antibody-antigen
precipitates produced by the precipitin reaction. Once they had isolated pure antibodies and examined their nitrogen content
and other chemical properties, they concluded that they were, in fact, a type of globulin, a group of proteins not soluble
in water but soluble in salt solutions.
Item is a photocopy.
Number of Image Pages:
12 (1,019,451 Bytes)
1936-08 (August 1936)
Kendall, Forrest E.
Periodical: Heidelberger, Michael, and Forrest E. Kendall. "Quantitative Studies on Antibody Purification: I. The Dissociation of
Precipitates Formed by Pneumococcus Specific Polysaccharides and Homologous Antibodies." Journal of Experimental Medicine
64, 2 (August 1936): 161-172. Article. 12 Images.
[Rockefeller Institute for Medical Research]
Reproduced from the Journal of Experimental Medicine, 1936, 64, 161-172 by copyright permission of the Rockefeller University
Medical Subject Headings (MeSH):
Antigens and Antibodies: Heidelberger and The Rise of Quantitative Immunochemistry, 1928-1954