I want first of all to tell you how deeply I enjoyed working with you and that I am deeply indebted to you for all the time
and attention you gave me. My stay there was so profitable to me materially and spiritually that I wish I could return there.
Of course, I also hope that I would be able sometime to have you and/or members of the group here. Thanks again for so much.
I got your note and the survey of my preparation it was all due to your expert supervision that that it came out so well.
Fritz Lipmann wanted to try out Poly U and asked me about the preparation of the phosphorylase and I felt that the best I
could do was to defer him to the originator and I hope this did not cause you any inconvenience. I am sure your purchase
is going to be heavily in demand.
As regards[?] the use of pyridinium Dowex so for leaving[?]KOH and all K+ ions from hydrodyates[?] , this is very simple.
We first clean the commercial Dowex 50 (H+) by NaOH then excess of HCl cycle -- till as NaCl lows OH -- wash thoroughly with
[ . . . ]and keep it in this acidic form. With passage of time this resin releases some low mol. wt. colored polymers and
when I came to using it for making Py+form we pack some mls of web[?] resin in a little column and put 10-20% Pyridaie[?]
through it and wash it for 1/2 hours or so with water. Russell did it while I was there. This freshly prepared pyridiumum
(Py+H) resin can be used either in trained form by adding it to the KOH hydropate[?]
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or by passing the KOH hydroypate though a column of this resin and washing the column[?] with 51% of Pyridine[?] For 1 mole
of KOH ca 2-3 ml. of resin is more than enough. Excess of resin doesn't matter of course. The eluate from column now
contains the [ . . . ] as pyridine salts and pyordine[?]. If you wish to concentrate it by evaporation, pyridine[?] goes[?]
OH first pH is likely to drop down to about 3. Use this as solution for any purpose you want.
For small scale, as I suggested to Leon, we could add Py+H resin to the alignds[?] of alkaline objects and spin down the resin
- - (it packs quite-nicely) and use supernate for analysis. If there are any questions let me know.
Separately I am sending a copy of my little book. Hope you find something of interest in it.
With all the best of regards to you and Dan and love to the children.