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The Maxine Singer Papers

Letter from Maxine Singer to Robb E. Moses pdf (113,202 Bytes) transcript of pdf
Letter from Maxine Singer to Robb E. Moses
Number of Image Pages:
2 (113,202 Bytes)
1970-04-22 (April 22, 1970)
Singer, Maxine
Moses, Robb E.
Harvard Medical School
Original Repository: Library of Congress. Maxine Singer Papers
Reproduced with permission of the Library of Congress.
Exhibit Category:
Nucleic Acids, the Genetic Code, and Transposable Genetic Elements: A Life in Research
Box Number: 4
Folder Number: 1
Unique Identifier:
Document Type:
Letters (correspondence)
Physical Condition:
Series: Correspondence, 1955-2004, n.d.
SubSeries: Chronological
Folder: 1970 January-June
April 22, 1970
Dear Robb:
Your query about Art Buchwald took me by surprise. I have no idea about any hidden identity of his.
I've been thinking about answering your long letter but that of course does you little good. In fact I over-committed myself to all sorts of things this spring and you as well as others have suffered (me too -- exhaustion and dissatisfaction with not being at the bench).
We were of course terribly lucky with the manuscript -- and I'm glad. There have been a spate of papers from Paris -- half-baked with respect to data but with good ideas and probably right. It's nice to have the three papers with their really elegant data to counteract this (Chou & Moses). One of the papers has extensive data on the lag in polymerization with independent E. coli enzyme -- overcoming it with miniscule amounts of primer. Actually they conclude that coli enzyme (even independent) has an absolute requirement for primer. On the face of it we seem to have switched places. Actually it was rather easy to demonstrate the same lag with both form-I and form-T using the new assay (remove ADP with charcoal and then use Ames phosphate method which increases sensitivity enormously and allows one to measure 6 and 10 n moles of Pi per 50 u liters of reaction with no trouble). In fact, this same lag was what was giving me trouble with my high s. a. ADP incorporation assay last spring when I tried to do the careful Km values for ADP and Mg. At the time I ascribed the trouble to the assay but the lag was real. BUT -- this lag is a phenomenon of insufficient free Mg2+ and disappears if the free Mg2+ is high enough. One can calculate from the Parisienre experiments that they were working at suboptimal free Mg2+ (using their own old data) so who knows what the lag means. One thing it does mean is that I can't do all those fancy Km values. That's really fine with me since it's a bore at best.
You are, of course, at liberty to talk about phosphorylase. Do you have the slides you need?
Now to the main point. The word here is that jobs are scarce. Independent slots probably next to impossible. I assume you want some degree of independence at this point. But it would help me if you gave a clearer idea on your views vis-a-vis independence and further and feelings about what field you're interested in for an independent project.
It is my general impression that there are certain NIH spots which are not as tight as others and furthermore they may be the sort of situations where people would be very glad to have someone with your background. By and large I would not term these "independent" but they might be the sort of situations where within your own problem you might be essentially on your own. Several situations may be worth investigating. One is Salzman -- one is the area of animal viruses (tumor viruses) and one is interferon. All of these do have the sort of clinical implications you mention. I'd be happy to do same phoning around if you'd like me to and then I'll report back in detail. Let me know. No rush -- I leave Saturday (April 25) for three weeks (India) and will be back about May 15.
I had a visit in Chicago. Jim is really blooming -- it was a pleasure to see.
All for now.
Best to Audrey.
Maxine Singer
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