Thank you so much for your nice letter! Such exciting developments in the lab! Actually, I had heard of the complexity of
f 1 histone with superhelical DNA. Betty Keller told me, and she had heard this from Joan Steitz for the first time last
month. Gordon Tomkins and others were trying to recruit the Steitz's and I just happened to be passing through. Last
week both of them gave a seminar at Cornell. Please send me a preprint to use for my lecture on DNA -- histone interactions.
Gary Felsenfeld sent me some stuff and a letter. This may be the end of my nucleic acid lectures. It's a bit silly to
attempt to keep up in this field when my own work is so remote, and people like Jeff Roberts are available to do the job.
The cell-free system for SV-40 DNA sounds exciting also, but I lack back ground information. Do you mean that LeBlanc has
something comparable to an RFII replication system in phage, for example?
We're in Boston for a long weekend. A project site visit on Thursday night and Friday night until 315 pm. Visits to
Boston Museum Friday 330 - 530pm. Visits to the Gardner Museum on Thursday afternoon. Tomorrow (Saturday) another visit
to Boston Museum, concentrating this time on Oriental art. Then lunch with the Kalckans. In the afternoon, perhaps a visit
to Cambridge. A concert in the evening -- the Boston Symphony. Sunday morning, a
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visit with the Khoranas. Then, Adelaide and I fly home again. It's awfully nice to see this area again -- I'm almost
sorry that Alan graduated, and left Harvard. Yale is O.K., but nothing great. The museum is very nice -- quite the best
college art gallery, but the New Haven downtown area is faintly unpleasant and Lake Place, Alan's street, is [ . . . ]
by. We do enjoy driving to Litchfield and other places outside New Haven. We enjoyed visiting in the Cavellakis home, but
that is out now. So, when Alan returns to Yale, we look forward to visits, but only to see Alan.
We leave for England about Mar 15. I'll be working with Dr. Stoker, but I don't have any good ideas as to what might
usefully be done in a period of 6 months. Except to catch up on plays, opera and ballet. Alan is looking for an apartment
for us. (David, Monica and Jenny are O.K. and very busy).
Work in the lab is going rather well. The most exciting development concerns a reconstitution of ATPase sub units by Jeff
Smith (post doc) and Paul Stern was (graduate student). Let me explain the back ground. The E. coli membrane ATPase, or
cupline factor ColiF1, consists of 5 subunits. The complete enzyme will reassociate with depleted membranes, adding on to
specific birding sites. In this way important functions are reconstituted, such as DCCD sensitivity, oxidative phosphorylation
and ATP-driven transhydrogenase reaction. Certain fractionation schemes yield a 4-subunit enzyme, which will not bind to
depleted cytoplasmic membranes to reconstitute these functions. Ef Racker, Nelson and others have sought to isolate the smaller
ATPase subunits (P and E), keep them viable, mix them with bigger subunits and reconstitute a totally recompetant coupling
factor. These efforts have not been successful. Smith and Sternweiss, with small modification of an old Racker procedure
have now succeeded. So were in the middle of a busy and exciting field. Best to Dan.