Letter from Marianne Grunberg-Manago to Maxine Singer
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12 October 1958
Institut Biologie Physico-Chimique
Original Repository: Library of Congress. Maxine Singer Papers
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Medical Subject Headings (MeSH):
Nucleic Acids, the Genetic Code, and Transposable Genetic Elements: A Life in Research
Letter from Maxine Singer to Marianne Grunberg-Manago (October 14, 1959)
I am already in Paris since 2 weeks, but I had to do many things which are waiting in the lab that I just got now to read
carefully our paper -- I think more and more that the inhibition[?] of poly A polymerisation or exchange by poly U and vice
versa is due to the interaction of poly A and poly U. I heard Severo discussing Poly phosphorylase at Vienna and saying that
the prime effect of polynucleotides is specific. His evidence is that poly A formation is not stimulated by poly U and vice
versa, but they are both stimulated by poly C and this is the only evidence for specificity. It could be better explained
by the interaction of poly A with poly U -- and I don't think that he really has an evidence for specificity. It seems
to me that it is really an important point to test the inhibition[?] of the enzyme by the multi stranded configuration.
I wonder if you did more experiments to test this point -- your data of [ . . . ] the enzyme with the complex poly A poly
U seemed to be [ . . . ] grief. Instead of having an additive effect by adding the complex to GDP you have an inhibition[?]
of 30%. -- (0.11 GDP alone 0.08 GDP complex 0.004 complex)
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[BEGIN PAGE TWO]
Going back to the paper, I think it would be fine if the phosphorysis data are included.
Just some little things
p. 6 last line at a GDP/pi ratio of 0.5 and 0.01MMgcl2 I think it is 0.001 MMGCle.
p. 7 Discussion of dinucleotide[?] stimulation. Didn't we conclude that there is a stimulation when we use little enzyme
and now stimulation when we use more. If not, I think we can just say that the results are inconsistent but the stimulation
was never up (not go through all the results)
p. 8 may be emphatize[?] that all the 3' ended gave a stimulation as big as the 5' ended if not bigger
p. 11 It seems that there is a stimulation exchange of P32 with TRPP by poly U is it constant? Certainly the stimulation
is only 15% but it might be significant.
Particularly that the exchange with TRPP seems very low and if I remember will the exchange with coli enzyme is as good with
TRPP with ADP.
I am writing now the paper on phosphorolyis and I will send it to you and Leon as soon as I write it. Doty suggested to publish
it on the new journal "Molecular Configuration" what do you think will it be a good journal?
How was the meeting in New York? What is new? Have you seen Severo and discussed with him?
Long asked me to write the description of polynucleotide phosphorylase and "dexynucleoside[?] trans[?] deoxyrelosyslase
[?]" Has[?] with enzymbe Broch J 1952 for a Biochemist Hand book I wrote already about but I don't formed[?] anything
else for the last enzyme that the two has [?] with papers. Do you know by[?] or Leon knows something more recent on this
I am very luck[y] to have Alice del Campeleo now Alice Campbell with me this year and she already started the purification
of yeast enzyme.
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I don't think there was really something new in Vienna and the high polymer symposium looks pretty dull to me. The protein
symposia was nice and I enjoy very much the one on virus. I don't remember if I wrote to you about Bert Grirer in Schamm's[?]
laboratory gave a nice talk. He found that he can treated infective TMV RNA with nitrones[?]and[?] and desamminate[?] the
bases. The RNA is still ineffective but it produces virus mutant. He can treated the TMV RNA after isolation or just treated
the virus and isolate the RNA after in both case, the RNA produces mutant viruses.
I wrote a letter to our friend Jeau[?] Claude and[?] Medeline Gautier.
Catherine and Amaud[?] are fine. Amaud[?] has now an exhibition in NY. "Niveau Galeine[?]" Madison Av and 74 St
-- I believe -- the exhibition will be until the 23rd of October -- if you will be in N.Y. Could you go and tell us how it
was? I enjoyed to see Earl and Teny in Paris. We went shopping with Teny through all the shops in Paris.
Please give my best regards to all my friends particularly to Russ Leon and Bernie -- when is he going to N.Y.?
But regards to tour husband [ . . . ] and to you
P.S. Can Russ send to me [ . . . ] and
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All the instructions for growing cells and purifying the enzyme -- By the way did you repeat the purification? The [ . .
. ] send you their best regards.