Short Notes on a Course of Practical Physiology by Dr. Burdon-Sanderson at University College London, 1872-73
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7, 10-11 October 1872
Original Repository: Osler Library of the History of Medicine, McGill University. Bibliotheca Osleriana
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Medical Subject Headings (MeSH):
Medical Education and Early Career, McGill University, 1870-1884
Short Notes on a course of Practical Physiology
Wm. Osler, M.D.
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Examination of inflamed anterior chamber of eye of frog and of lymph sac. They were prepared in the following way. A thread
was passed into the anterior chamber and through the cornea, one end then cut short and pulled within the other like wire.
A portion of frogs skin soaked in ammonia was then inserted into the lymph sac over the cervical region and pushed down towards
the lumbar and there left. The animal is ready for examination in two days. To remove the pus from eye, prick it with a [triangle]
knife and then quickly insert a capillary pipette. Numerous pus globules are found, all of them exhibiting very active movements,
changing shapes rapidly, containing granules, vacuoles, and a nucleous.
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The cornea is apparently not much altered but when examined in the fibrous stroma between the epithelial layers are found
"wanderers" exhibiting movement. Treat the lymph sac in following way, dissect carefully off a thin year, taking care
not to touch the endothelium (and it is best done under water) and brush it with a solution of AgNO3 [silver nitrate] 1/4
percent, then place it in the sunlight for a short time, on examination it will be found that the outlines of the cells are
stained and the pus globules are coloured.
10th -- Examination of omentum of rabbit 3 days after injection with ammoniated milk. After being brushed with solution of
AgNO3 and exposed to light, cut square pieces and examine, floating them onto the glass slips and taking great care to obtain
the specimen free from
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folds and creases. The serous epithelium is seen mapped out by the AgNO3, the cells containing many minute particles looking
like fine oil globules, but may be the deposit of AgNO3 in them. Vessels of three kinds are seen, veins, arteries, and lymphatics,
the latter being the interesting ones. They seem to be found more in the track of the vessels than in the interspaces between
them and have their endothelium well brought out by the staining.
11th -- Continued examination of inflamed and health omentum of rabbit. The lymphatics as shown by the AgNO3 method present
an imbricated appearance due to the stained outline of the endothelium. On serious surfaces generally the relation between
parts is supposed by recent observers to be as follows: Beneath the epithelium ramify
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the lympatics and capillaries together with a system of branched nucleated cells which communicate with both the lymphatics
and the adjacent ones. Opening onto the serous surfaces are certain bodies called stomata, of which there are two kinds, the
true and the false.