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The Martin Rodbell Papers

Title:
Letter from Martin Rodbell to Peter F. Hall pdf (79,956 Bytes) transcript of pdf
Letter from Martin Rodbell to Peter F. Hall
Description:
Rodbell, writing in response to a request for a reprint of one of his articles from the series he published with Stephen L. Pohl and Lutz Birnbaumer, outlined in this letter some of their thinking on the role of guanine nucleotides in hormone action at the time the article was printed and how it has evolved since.
Number of Image Pages:
1 (79,956 Bytes)
Date:
1979-11-30 (November 30, 1979)
Creator:
Rodbell, Martin
Recipient:
Hall, Peter F.
Rights:
This item is in the public domain. It may be used without permission.
Subject:
Medical Subject Headings (MeSH):
Adenylyl Cyclases
GTP-Binding Proteins
Guanine Nucleotides
Hormones
Exhibit Category:
Signal Transduction and the Discovery of G-Proteins, 1969-1980
Relation:
Metadata Record The Glucagon-Sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver: I. Properties (March 25, 1971) pdf (1,195,715 Bytes) ocr (39,101 Bytes)
/ps/access/GGAAAW.pdf
Metadata Record The Glucagon-Sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver: II. Comparison Between Glucagon- and Fluoride-Stimulated Activities (March 25, 1971) pdf (501,891 Bytes) ocr (16,814 Bytes)
/ps/access/GGAAAX.pdf
Metadata Record The Glucagon-Sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver: III. Binding of Glucagon: Method of Assay and Specificity (March 25, 1971) pdf (1,971,973 Bytes) ocr (63,171 Bytes)
/ps/access/GGAAAY.pdf
Metadata Record The Glucagon-Sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver: IV. Effects of Guanyl Nucleotides on Binding of 125 I-Glucagon (March 25, 1971) pdf (927,809 Bytes) ocr (29,777 Bytes)
/ps/access/GGAAAZ.pdf
Metadata Record The Glucagon-Sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver: V. An Obligatory Role of Guanyl Nucleotides in Glucagon Action (March 25, 1971) pdf (892,231 Bytes) ocr (29,478 Bytes)
/ps/access/GGAABA.pdf
Box Number: 4
Folder Number: 5
Unique Identifier:
GGAABE
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Series: Correspondence, 1961-1998
SubSeries: Arranged by Year, 1961-1998
Folder: 1979
Transcript:
November 30, 1979
Dear Mr. Hall:
I am glad to hear that someone is still interested in an "ancient" paper of mine. That paper, as you know, is one of the first in a series related to the role of guanine nucleotides in hormone action on adenylate cyclase systems in membranes. As the first in a series, we naturally followed up many of the questions raised in this study. We know now, for example, that GTP contaminates both the membrane preparations and is a major contaminant in some commercial preparations of ATP. When these preparations are cleaned of contaminating GTP, hormone action does not occur without the addition of GTP. Our attempt to settle the question in the first paper was to incubate the membranes for a period of time prior to assay in order to "digest" any contaminating GTP in the membrane preparation. That experiment was not really conclusive; nonetheless, Fig. 5 gave us some feeling that GTP was important if not essential to the action of glucagon. Now we know that GTP and glucagon interact with separate protein molecules which are joined functionally to give a unit we termed RN (R for receptor, N for nucleotide regulatory protein). The N unit is responsible for activating adenylate cyclase when liganed with GTP. The receptor seems to inhibit the ability of N to interact with GTP; binding of the hormone to R relieves this inhibition, allowing GTP to bind to N. Thus, N becomes activated by GTP and then combines (along with R) with adenylate cyclase to form the activated enzyme. This, in a grossly simplified form, is the status of the problem after nearly nine years of research. It goes on. Hope you and the others become interested in the problem. It still fascinates me.
Sincerely,
Martin Rodbell, Ph.D.
Chief, Laboratory of Nutrition and Endocrinology
Metadata Last Modified Date:
2010-04-12
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