Subject: Sixth Quarter Report for the period July 1, 1965 to September 30, 1965
1. One shipment of fish was received from Africa and one quite large specimen was in excellent condition.
2. Four (4) glass aquaria were purchased and were fitted with acrylic plastic tops especially designed for oxygen consumption
studies of fish.
3. A fish weighing 1 pound and 9 ounces was studied during a quiet but active state and on two separate 24 hour occasions
his oxygen consumption was as follows:
(a) Run A - 26 hr. and 20 mins. 88ml
(b) Run B - 24 hours exactly 104ml
This fish was subsequently forced into estivation by removing the water from the tank. He succeeded in entering estivation
but with so markedly enlarged air content of the system, accurate oxygen consumption studies could not be taken of so large
a fish. The fish survived two weeks of estivation and then succumbed.
4. Four (4) large fish are currently in the process of enforced estivation.
5. The final draft for a paper entitled "Oxyhemoglobin Dissociation Curve in the Blood of Lungfish" has been transmitted
to the American Journal of Physiology for publication.
6. In view of the remarkable and encouraging report of Axelrod at the Third International Symposium on Hybernation of finding
a hybernating property in crude extracts from the brains of the hybernating ground squirrel, we will soon enter intensive
study of extracts of the brains of the 4 fish that are currently estivating and of others to be imported. Other interesting
and pertinent papers were given at this Conference which was attended by the principal investigator.
In summary, definite progress was made during the quarter and a lead has been obtained which promises a rewarding course of