The Dependence of Cell-Free Protein Synthesis in E. Coli Upon Naturally Occuring or Synthetic Polyribonucleotides
In this landmark essay, published in conjunction with "Characteristics and Stabilization of DNAase-Sensitive Protein Synthesis
in E. coli Extracts," the authors present the same conclusions revealed in Nirenberg's address at the now famous International
Congress of Biochemistry in Moscow during August 1961. Adding an artificial form of RNA, polyuridylic acid, consisting of
the base uracil, to the E. coli extract resulted in the production of an unnatural protein composed entirely of the amino
acid phenylalanine. The experiment showed that UUU was the codon for phenylalanine and that messenger RNA, which transcribes
genetic information from DNA, directs protein synthesis. This provided a route to base compositions of codons and the exploration
of the general nature of the code.
Number of Image Pages:
15 (1,644,825 Bytes)
1961-10 (October 1961)
Nirenberg, Marshall W.
Matthaei, J. Heinrich
Periodical: Nirenberg, Marshall W., and J. Heinrich Matthaei. "The Dependence of Cell-Free Protein Synthesis in E. Coli Upon Naturally
Occuring or Synthetic Polyribonucleotides." Proceedings of the National Academy of Sciences of the United States of America
47, 10 (October 1961): 1588-1602. Article. 15 Images.
National Academy of Sciences (U.S.)
Reproduced with permission of Marshall W. Nirenberg.
Medical Subject Headings (MeSH):
Synthetic RNA and the Poly-U Experiments, 1959-1962
Characteristics and Stabilization of DNAase-Sensitive Protein Synthesis in E. Coli Extracts (October 1961)