Cell-Free Protein Synthesis Directed by Messenger RNA
Nirenberg's work with synthetic RNA and E. coli provided the basis for his genetic research in the early 1960s. This
article describes the preparation of reagents, procedures for reaction mixtures, methods for growth of E. coli, and preparation
of extracts. Their experiments ultimately proved that messenger RNA, which transcribes genetic information from DNA, directs
protein synthesis. That is, messenger RNA transmits the DNA messages that prescribe the assembly of amino acids into the complex
proteins that drive living processes.
Item is a photocopy.
Number of Image Pages:
7 (610,010 Bytes)
Nirenberg, Marshall W.
Periodical: Nirenberg, Marshall W. "Cell-Free Protein Synthesis Directed by Messenger RNA." Methods in Enzymology VI, (1964):
17-23. Article. 7 Images.
Reproduced with permission of the Academic Press.
Medical Subject Headings (MeSH):
Translating the Code of Life and the Nobel Prize, 1962-1968