Letter from Michael Hanley to Marshall W. Nirenberg
Hanley updates Nirenberg on the progress of research, using cell lines obtained from Nirenberg earlier, in the Molecular Neurobiology
Unit at the University of Cambridge Medical School. Hanley also requests additional clonal cell lines for future study.
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1986-12-09 (December 9, 1986)
Medical Research Council of Great Britain
Nirenberg, Marshall W.
Courtesy of Marshall W. Nirenberg.
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To keep you up to date on our progress with the cell lines, we have now started sub-cloning by limiting dilution each of the
populations you sent to us. It seems likely that most, if not all, of the NCBS and NCFL series are oligoclonal. The resulting
sub-cloned lines are being sent to Anna Borsodi for her programme as well as being evaluated here. Nothing very interesting
has yet emerged.
On another matter, we should still be very grateful if the clones for the G-proteins were to be made available to us. We
should be delighted to receive anything we could, but the clones for Gs and Gi were muted, and we should be particularly interested
if a Go probe were also possible.
Thank you for your help. Best wishes, and Merry Christmas,