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The Marshall W. Nirenberg Papers

Title:
Laboratory project: "Muscarinic Acetylcholine Receptors of Cultured Cell Lines" pdf (120,028 Bytes) transcript of pdf
Laboratory project: "Muscarinic Acetylcholine Receptors of Cultured Cell Lines"
Description:
Studies on muscarinic acetylcholine receptors focus both on ligand-binding and on defining the physical properties of muscarinic receptors.
Item is a photocopy.
Number of Image Pages:
2 (120,028 Bytes)
Date:
1979-09 (September 1979)
Creator:
Nirenberg, Marshall W.
Kline, William L.
Hurko, Orest
National Heart, Lung, and Blood Institute. Laboratory of Biochemical Genetics
Rights:
This item is in the public domain. It may be used without permission.
Exhibit Category:
From Neuroblastoma to Homeobox Genes, 1976-1992
Relation:
Metadata Record Annual Report of the Laboratory of Biochemical Genetics, October 1, 1978 - September 30, 1979 (September 1979)
Box Number: 6
Folder Number: 22
Unique Identifier:
JJBBQG
Accession Number:
2001-015
Document Type:
Reports
Excerpts
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Series III: Laboratory Administration, [1959]-1993
SubSeries: Annual Reports, 1960-1993
Folder: Annual Reports, 1960-1988
Transcript:
Project Number: Z01 HL 000012-05 LBG
Period Covered: October 1, 1978 - September 30, 1979
Title of Project: Muscarinic Acetylcholine Receptors of Cultured Cell Lines
Names, Laboratory and Institute Affiliations, and Titles of Principal Investigations and All Other Professional Personnel Engaged on the Project:
PI: Marshall Nirenberg, Chief, LBG, LBG NHLBI
Other: William L. Kline, Guest Worker, LBG NHLBI
Orest Hurko, Staff Associate, LBG NHLBI
Cooperating Units (if any): None
Lab/Branch: Laboratory of Biochemical Genetics
Section: Section on Molecular Biology
Institute and Location: NHLBI, NIH, Bethesda, MD 20205
Total Man Years: 0.25
Professional: 0.25
Other: 0
Summary of Work: Studies on: muscarinic acetylcholine receptors focus both on ligand-binding and on defining the physical properties of muscarinic receptors.
Project Description:
Major Findings: [3H]-Quinuclidinyl-benzilate (QNB) was used to study muscarinic acetylcholine receptors in NG108-15 membrane preparations. The apparent dissociation constant of [3H]-QNB is 1 x 10^(-10) M; the average NG108-15 cell possesses 30,000 specific sites for [3H]-QNB. Activation of the receptors with acetylcholine or carbachol results in cell depolarization, a small increase in cellular cGMP, and inhibition of adenylate cyclase. Cell depolarization and rise in cGMP levels desensitize in 30 sec; whereas, the inhibition of adenylate cyclase does not desensitize. Scatchard analysis revealed only one homogeneous class of [3H]-QNB binding sites; however biphasic rates of [3H]-QNB association with and dissociation from receptors were found. Evidence was obtained for the formation of a dissociable [[3H]-QNB Receptor] complex which then is converted to a form which dissociates only slowly. Hill coefficients of approximately 1.0 were found for receptor antagonists and approximately 0.5 for receptor activators. A sequential series of reactions were proposed to account for these observations and for the various states of the muscarinic acetylcholine receptor that were detected.
Publications:
1. Burgermeister, W., Kline, W.L., Nirenberg, M., and Witkop, B., Mol. Pharm. 14, 751-767 (1978).
2. Hurko, O. Specific [3H]-Quinuclidinyl Benzilate binding activity in digitonin-solubilized preparations for bovine brain. Arch. Biochem. and Biophys. 190, 434-445 (1978).
Metadata Last Modified Date:
2010-09-20
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