Green requests some of Nirenberg's neuroblastoma cells for research in the Department of Biology and Preclinical Medicine
at the University of St. Andrews in Scotland. He offers to obtain the cells from Dr. Bradford at the Department of Biochemistry
at the Imperial College of Science and Technology in London, rather than having them sent from Bethesda, MD.
I am writing to request a gift of some of your neuroblastoma clone NS-20Y. We may be able to obtain this line with permission
from Dr Bradford at the Department of Biochemistry, Imperial College of Science and Technology, London, where you have also
donated them. This might be more convenient than sending them from the USA.
We are currently starting a new research project. I enclose the relevant pages of our grant application. We originally planned
to use the astrocytoma clone D384 as a source of D1 dopamine receptors. We have subsequently found, however, that NS-20Y
cells also contain D1 receptors and may be a better cell line to use (Monsma and Sibley, 1988, Soc. Neurosci. Abs. 14, 165.12).
This will enable better comparison with our source of D2 receptors (NCB-20) and should hopefully enable monitoring of both
channels by electrophysiologioal means in the Xenopus oocytes.
With your permission we will contact Dr Bradford and request some of their NS-20Y cells.