Letter from Haruhiro Higashida to Marshall W. Nirenberg
Higashida updates Nirenberg on his research project on hybrid cells at the University of Kanazawa School of Medicine in Japan.
He provides Nirenberg with abstracts of work and data from experiments. This letter concludes with a request for more cells,
qualified by the statement "these cells will only be used as a part of my own experiments."
I'm spending a very busy time, because every tasks are quite new to me as a laboratory head, though these are not distressful.
Unfortunately I haven't had an invitation letter from the Cold Spring Harbor Laboratory for this year's symposium
on Molecular Biology on Signal Transduction, I have two abstracts in the meeting as a coauthor with S. Numa and D. Brown.
Two abstracts are enclosed for your interest.
The research project on N x L hybrid cells are going well. The enclosed is a piece of data which shows the IP3(1,4,5) formation
is a signal for ACh-induced hyperpolarization. We are planning to define which muscarinic subtype is present in these NL
(308, 309, & 1F) hybrid cells.
As you know Toshi Nagastu has cloned the tyrosine hybroxylase gene, he is interested in seeing the gene expression of species-difference
in clonal cell lines as well as different tissues. I've provided him N1E115 and NX31T cells which really have TH molecules
in them (see enclosed photos). I remember that you said you have several adrenergic clones of chick retina and neuroblastomas
Please send me N18-RE-105 cells and if possible, one or two other retina hybrid cells in your list for TH-gene-expression
experiments, under the understanding that these cells will only be used as a part of my own experiments participated.